Please tell me the best PBS recipe for both cell culture and super resolutionmicroscopy?

When ever I mount the cover slips on the slides to perform the microscopy I find crystals on the other side of the cover-slip after drying. please suggest me the better recipe where i can use it...

04 May 2019 4,263 5 View

Can any one suggest me the best ratios for fugene transfection with HEK293 cells?

after transfection with fugene reagent i can see most cells are dying. please suggest me a good practice protocol to get best transfection and better ratios. need regular method used in the lab.

01 February 2019 9,084 2 View

Do you want to participate in national symposium on current research in cancer biology & therapy?

06 July 2016 8,129 0 View

Why do we use wbc chamber in hemocytmeter for cancer cell lines count when ever we split the cell why dont we use RBC chamber?

give me a better answer only size is the major reason or is there any other restrictions or can we use RBC chamber also.

06 July 2016 5,685 2 View

Is there any evidence that animal plant cell fusion and successful revival?

because i want to know regarding plant cytoplasam and animal cytoplasam differences?

06 July 2016 5,103 1 View

Why do we use methanol in western blotting? and why do we use methanol for pvdf membrene activation?

please answer me with detailed chemical reaction. thanking you.

06 July 2016 366 4 View

Can anyone provide me with a definition of liveliness?

We are trying to propose the relation between animal psychological behavior with it's DNA for my dram project. Regarding this I've produces me own definition liveliness. Can anyone provide me with...

02 March 2015 906 4 View

What is livingness? How it differs in a moving architecture and non moving architecture of living thing?

i need some details to confer it. please share and help me

01 January 1970 4,008 0 View

Which type of material used for making animal cell culture plates ? which material able adhere to with animal cells growth factors?

I need the material used in making and how that material can form bond with growth factors.

01 January 1970 3,328 3 View

I want to establish t cell culture in my cancer cell lines lab can any one suggest me the better and good way to start my work?

01 January 1970 8,312 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View