I need to isolate nucleus from budding yeast with high purity. I need intact nucleus. Can someone suggest any protocol that I can use?

30 December 2017 0 4K Report

I need high purity nuclear fractions from budding yeast cultures for biochemical assays. I am able to get spheroplasts but getting the nucleus out without damaging the nucleus and without homogenization looks difficult. But can anyone suggest any protocol.

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