I have a Sanger sequences for 8exon of genes, I want to detect the mutation on those exon and the effect of this mutation on the protein , how?

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What would you do next?

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Katie A S Burnette

Just find an online Translation tool and tell it which reading frame to use.

Frederic Lepretre

Hi Najwa Alharbi

you'll need a tool as VEP from Ensembl (https://www.ensembl.org/info/docs/tools/vep/index.html) to predict the effects.

all the best

fred

Berjas Abumsimir

Use the Nagahama mutation finder server or another one. Just verify your raw results by NCBI basting before.

Najwa Alharbi

thank you all for your answers, would you please give more details, I am new to this area