What is the most suitable NMR solvent for carbon-based materials?

What is the most suitable NMR solvent for carbon-based materials?

25 December 2020 5,020 4 View

Effect of adsorbent porosity on efficiency of adsorbent recovery ?

Hi guys. Can anyone answer the following with citing the references? 1) Relationship between adsorbent porosity and adsorption/desorption rate? 2)Relationship between porosity and adsorbent...

31 October 2020 6,323 5 View

Where is the best location for placement of a magnetic filter in a amine system?

One solution which is presented for eliminating suspended Iron sulfide (FeS) contamination from refinery amine system, is using from magnetic separation. where location of amine circulation (rich...

09 September 2020 9,879 6 View

What is teaching? rethinking about concept of teaching after coronavirus?

every person knows that teaching is the most important weapon we can use change the world. but there is an important question which education ? or teaching? when I think this question my mind...

04 August 2020 6,998 3 View

What can be joint projects between chemistry and mechanical engineering?

how can I find a common related project between chemistry (polymers or biomolecules) and mechanical engineering( for example fluid mechanics )???

15 April 2020 2,388 1 View

Between intrinsic catalytic properties and Electrochemically active surface area, which of them is more important to enhance catalytic activity?

dear all, We know that the catalytic activity of any materials depend on their intrinsic catalytic activity and their active surface area, I want to know, which of them is more important to...

16 January 2019 2,601 1 View

What is the appropariate property method for simulation a process contains component such MeOH, H2O, and hydrocarbon olefins?

I'm need to select a property method for estimation physical property of above mentioned components that involved in a chemical process at high temperature and moderate pressure. in the most parts...

05 August 2018 3,473 5 View

How can apply a lumped kinetic model in simulation software such as Aspen plus?

In Some kinetic modeling articles, a reaction network has been proposed , where in some components with similar properties ( but different molecular formula and structure), put in a hypothetical...

17 October 2017 9,408 6 View

What is the best protocol for ihc stainng to detect GluR2 (subtype of AMPA reseptor)?

14 October 2016 6,718 5 View

How can I to measure growth speed in plants?

11 May 2016 9,637 6 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View