Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?
Hi all, I mean chromogenic methods.
15 June 2024 4,680 0 View
Hello everyone I am trying to simulate a structure in Abaqus-Hypermesh insterface, considering the half of the model due to a symmetry. The model is in 3D and I'd like to know how can I acquire...
11 April 2024 1,868 0 View
I found vWF, PAI1 and FVIII that are produced by endothelial cells, but I think there must be more, maybe more important factors to mediate coagulation process.
24 January 2024 6,165 3 View
Finally, I want to calculate free ammonium and for this purpose I need to know TAN.
29 October 2023 3,350 3 View
Is sum of two decreasing convergent sequence to 0, a decreasing convergent sequence to 0 in Archimedean Riesz space? If an and bn are o-convergent, is an+bn also o-convergent?
01 September 2022 4,093 2 View
we are casting grey cast Iron with DIZA machine. recently we have faced a new defect as a surface hole and the hole is surrounded by a thin layer of nodular graphite. the pictures from the...
31 August 2021 2,922 6 View
What is the most suitable NMR solvent for carbon-based materials?
25 December 2020 5,267 4 View
Hi guys. Can anyone answer the following with citing the references? 1) Relationship between adsorbent porosity and adsorption/desorption rate? 2)Relationship between porosity and adsorbent...
31 October 2020 6,537 5 View
One solution which is presented for eliminating suspended Iron sulfide (FeS) contamination from refinery amine system, is using from magnetic separation. where location of amine circulation (rich...
09 September 2020 10,071 6 View
every person knows that teaching is the most important weapon we can use change the world. but there is an important question which education ? or teaching? when I think this question my mind...
04 August 2020 7,184 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View