I confused about buffer exchange. which method is useful ?

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Fabian Schumacher

You may use a centrifugal filter unit to get rid of your phosphate buffer.

http://www.emdmillipore.com/CA/en/life-science-research/protein-sample-preparation/protein-concentration/amicon-ultra-centrifugal-filters/YKSb.qB.GXgAAAFBTrllvyxi,nav

Afterwards you can dissolve your retained DNA in the solvent of your choice.

Kind regards

Snigdha Maiti

You can try ethanol precipitation or ultrafiltration devices.

Ethanol precipitation can give good yield. Very small amounts of DNA can be recovered efficiently.

Whereas ultrafiltration devices work very fast in desalting and buffer exchange applications but it is less quantitative.

Another way is to lyophilize the sample, wash several times with 70% ethanol , dry and re-dissolve it in your choice of buffer. The resuspension process might take long incubation period.

Alexander B Westbye

I agree with Snigdha that precipitation is a good idea - especially if you have longer DNA fragments (eg. genomic or plasmid DNA). Isopropanol can also be used for precipitation, and there are many protocols online (eg. https://openwetware.org/wiki/DNA_Precipitation).