Hybridization of Oxidized nucleobase ?

More Manohar Raju's questions See All

6-Mercaptohexanol in DNA biosensor ?

Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...

04 May 2019 5,748 1 View

How would i fix a DNA probe concentration for a biosensor ?

Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...

02 March 2019 2,832 2 View

Electrochemical DNA Biosensor - LoD and LoB ?

Hi all, How one can calculate the LoD (Limit of detection) and LoB (Limit of Blank) for DNA based biosensor (Label Free). Should one has to take LoB after immobilizing the DNA probe...

31 December 2018 6,061 2 View

Oligonucleotides - NanoDrop Analysis ?

Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...

11 December 2018 593 2 View

Electrochemical Impedance Spectroscopy ?

How do they fit the Randle Sevick's Equivalent circuit for multiple data (Nyquist Plot). Since most of the journals shows a single equivalent circuit representing for multiple nyquist plot .

08 September 2018 5,756 5 View

No X and Y- axis values in CH electrochemical Workstation graphs ?

Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...

05 June 2018 5,523 3 View

Authorship for a Research Work ?

Hi, Most of the Institutes and Research centers has skilled staff who may be just matriculation passed, as they get experienced they would tackle any troubleshoot or problems they face. Most...

05 June 2018 4,952 3 View

How to quantify DNA immobilized on Electrode ?

Hi, Is it possible to quantify the amount of DNA that has been immobilized on the gold electrode ?. How to quantify using EIS/ Chronocoulometry or any other methods.

04 May 2018 9,097 3 View

Gold Nanoparticles Coated on Gold Electrode?

Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...

03 April 2018 1,474 3 View

How to perform Association and Dissociation constant, confirmation of Hybridization ?

1.How do i check the Kd (Dissociation Constant) and Ka (Association Constant) in DNA - DNA hybridization. 2.How can i confirm about the DNA-DNA Hybridization by experiments.

01 February 2018 8,971 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Guobing Xiang

Yes，for sure for 8-Oxo-Guanine ！

Manohar Raju

If yes, there could be change in free energy too.

any supportive materials.

Thanks Guobing Xiang

https://www.biosyn.com/oligonucleotideproduct/8-oxo-2-deoxyguanosine-oligonucleotide-modification.aspx#!

Manohar:

"8-oxo-dG can be incorporate into oligonucleotide at any position during synthesis ...." I believe there should be a lot of data out there, you just need to find it, I do not have excess here, sorry!

Thanks Guobing Xiang , will search for that .