Hybridization of Oxidized nucleobase ?

More Manohar Raju's questions See All

6-Mercaptohexanol in DNA biosensor ?

Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...

04 May 2019 5,937 1 View

How would i fix a DNA probe concentration for a biosensor ?

Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...

02 March 2019 2,987 2 View

Electrochemical DNA Biosensor - LoD and LoB ?

Hi all, How one can calculate the LoD (Limit of detection) and LoB (Limit of Blank) for DNA based biosensor (Label Free). Should one has to take LoB after immobilizing the DNA probe...

31 December 2018 6,223 2 View

Oligonucleotides - NanoDrop Analysis ?

Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...

11 December 2018 736 2 View

Electrochemical Impedance Spectroscopy ?

How do they fit the Randle Sevick's Equivalent circuit for multiple data (Nyquist Plot). Since most of the journals shows a single equivalent circuit representing for multiple nyquist plot .

08 September 2018 5,932 5 View

No X and Y- axis values in CH electrochemical Workstation graphs ?

Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...

05 June 2018 5,656 3 View

Authorship for a Research Work ?

Hi, Most of the Institutes and Research centers has skilled staff who may be just matriculation passed, as they get experienced they would tackle any troubleshoot or problems they face. Most...

05 June 2018 5,096 3 View

How to quantify DNA immobilized on Electrode ?

Hi, Is it possible to quantify the amount of DNA that has been immobilized on the gold electrode ?. How to quantify using EIS/ Chronocoulometry or any other methods.

04 May 2018 9,253 3 View

Gold Nanoparticles Coated on Gold Electrode?

Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...

03 April 2018 1,708 3 View

How to perform Association and Dissociation constant, confirmation of Hybridization ?

1.How do i check the Kd (Dissociation Constant) and Ka (Association Constant) in DNA - DNA hybridization. 2.How can i confirm about the DNA-DNA Hybridization by experiments.

01 February 2018 9,101 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Guobing Xiang

Yes，for sure for 8-Oxo-Guanine ！

Manohar Raju

If yes, there could be change in free energy too.

any supportive materials.

Thanks Guobing Xiang

https://www.biosyn.com/oligonucleotideproduct/8-oxo-2-deoxyguanosine-oligonucleotide-modification.aspx#!

Manohar:

"8-oxo-dG can be incorporate into oligonucleotide at any position during synthesis ...." I believe there should be a lot of data out there, you just need to find it, I do not have excess here, sorry!

Thanks Guobing Xiang , will search for that .