Hi,
In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the electrode.
- Most of them have used MCH and DNA in the ratio of 1000:1 i.e, 1mM MCH and 1μM DNA.
- whats the logic behind this 1000:1 ratio for MCH and ssDNA probe.
- Bare electrode coated with MCH also gives a peak when treated and washed with methylene blue, so how would one able to make out the presence of ssDNA probe on the electrode.
Hello Manohar Raju
To answer your first questions... altering the ratio provides some control of the packing density of the probe. This can help improve performance by reducing steric hindrance.
generally you will have to optimise this ratio depending on several factors (electrode material/structure, probe, spacer & any other component of the recognition layer, measurement buffer/matrix, and target).
You may find the following articles helpful, you will find details on the effect of altering the ratio:
Article Optimization of DNA immobilization on gold electrodes for la...
Article Self-assembled gold nanoparticles for impedimetric and amper...
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