21 Questions 34 Answers 0 Followers
Questions related from Manohar Raju
Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...
05 May 2019 5,886 1 View
Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...
03 March 2019 2,936 2 View
Hi all, How one can calculate the LoD (Limit of detection) and LoB (Limit of Blank) for DNA based biosensor (Label Free). Should one has to take LoB after immobilizing the DNA probe...
01 January 2019 6,169 2 View
Hi all, What are the possibilities of a oxidized nucleobase, getting paired with the complementary base. does it satisfies Chargaff rule ? Can 8-Oxo-Guanine be paired with Cytosine ?
01 January 2019 897 4 View
Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...
12 December 2018 692 2 View
How do they fit the Randle Sevick's Equivalent circuit for multiple data (Nyquist Plot). Since most of the journals shows a single equivalent circuit representing for multiple nyquist plot .
09 September 2018 5,878 5 View
Hi, Most of the Institutes and Research centers has skilled staff who may be just matriculation passed, as they get experienced they would tackle any troubleshoot or problems they face. Most...
06 June 2018 5,052 3 View
Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...
06 June 2018 5,617 3 View
Hi, Is it possible to quantify the amount of DNA that has been immobilized on the gold electrode ?. How to quantify using EIS/ Chronocoulometry or any other methods.
05 May 2018 9,210 3 View
Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...
04 April 2018 1,638 3 View
I have sequenced DNA data, now i would like to remove the primers from the sequence which is in fasta format. I have tried with BioEdit s/w, had aligned using clustalw and generated contig. In...
02 February 2018 9,437 7 View
1.Why there is a "Acid suffix" in RiboseNucleicAcid or DeoxyRiboseNucleicAcid. 2.Is it because of prescence of phosphoric acid ?.
02 February 2018 2,320 12 View
1.How do i check the Kd (Dissociation Constant) and Ka (Association Constant) in DNA - DNA hybridization. 2.How can i confirm about the DNA-DNA Hybridization by experiments.
02 February 2018 9,063 0 View
I had been for an Interview for a post of Research Fellow, the questions i had faced was. Qsn: Types of Primer Ans: I had replied as Degenerate Primers and Primers. The Interviewer was not...
01 January 2018 4,728 8 View
Hi, I have been working on Bacillus thuringiensis, when i amplify the Cry1 gene using genomic DNA i usually get a positive result. i need to know whether these Cry gene's are Genomic or...
10 October 2017 3,548 5 View
Looking for some Suggestions / Advice - may be a debatable question For a Researcher - Procuring Chemicals / Drugs always a burden since getting a Chemical / Peptide from In / Abroad in which one...
09 September 2017 192 2 View
Chemicals Grade based on Lower Grade(LR) , Analytical Grade(AR), Higher Analytical Grade(AR+) , Tissue Culture Grade, HPLC Grade, Molecular Biology grade. Analytical Reagent Grade Vs Molecular...
09 September 2017 2,023 4 View
Hi Everyone, Am looking for a Methods / Protocols for Electrochemical Biosensors, can some one shed the light on these aspects,since am new to Electrochemical biosensors , my aim is to build a...
09 September 2017 2,508 3 View
Hi Everyone, i have a Sequence of 16s rRNA, when i tried to generate CAP- Contig using BioEdit, am getting Multiple Contigs for a particular strain, but when i tried with some other strain am...
01 January 2017 2,816 3 View
Am using an ~ 6 years old Primers now, and am not able to get any amplicons, can this be due to degradation of nucleotides over time ? .
06 June 2016 1,520 11 View
Nanotechnology on Proteins for Larvicial activity and the equipments used for Nanotechnology.
05 May 2016 2,575 4 View
