Gold Nanoparticles Coated on Gold Electrode?

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6-Mercaptohexanol in DNA biosensor ?

Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...

04 May 2019 5,748 1 View

How would i fix a DNA probe concentration for a biosensor ?

Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...

02 March 2019 2,832 2 View

Hybridization of Oxidized nucleobase ?

Hi all, What are the possibilities of a oxidized nucleobase, getting paired with the complementary base. does it satisfies Chargaff rule ? Can 8-Oxo-Guanine be paired with Cytosine ?

31 December 2018 799 4 View

Electrochemical DNA Biosensor - LoD and LoB ?

Hi all, How one can calculate the LoD (Limit of detection) and LoB (Limit of Blank) for DNA based biosensor (Label Free). Should one has to take LoB after immobilizing the DNA probe...

31 December 2018 6,061 2 View

Oligonucleotides - NanoDrop Analysis ?

Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...

11 December 2018 593 2 View

Electrochemical Impedance Spectroscopy ?

How do they fit the Randle Sevick's Equivalent circuit for multiple data (Nyquist Plot). Since most of the journals shows a single equivalent circuit representing for multiple nyquist plot .

08 September 2018 5,756 5 View

No X and Y- axis values in CH electrochemical Workstation graphs ?

Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...

05 June 2018 5,523 3 View

Authorship for a Research Work ?

Hi, Most of the Institutes and Research centers has skilled staff who may be just matriculation passed, as they get experienced they would tackle any troubleshoot or problems they face. Most...

05 June 2018 4,952 3 View

How to quantify DNA immobilized on Electrode ?

Hi, Is it possible to quantify the amount of DNA that has been immobilized on the gold electrode ?. How to quantify using EIS/ Chronocoulometry or any other methods.

04 May 2018 9,097 3 View

How to perform Association and Dissociation constant, confirmation of Hybridization ?

1.How do i check the Kd (Dissociation Constant) and Ka (Association Constant) in DNA - DNA hybridization. 2.How can i confirm about the DNA-DNA Hybridization by experiments.

01 February 2018 8,971 0 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

Vadim Verlotski

Of course, you can polish off nanoparticles using polishing. You do not need a polishing agent for this, simple felt or cloth is enough. Couple of frictions go over it with felt and a couple of hundred nanometers of gold are gone! Do not rub too long, otherwise you have half your electrode eliminated .

Manohar Raju

Thanks for the Insights Vadim Verlotski.

Beddiaf Zaidi

I agree with Vadim