1.How do i check the Kd (Dissociation Constant) and Ka (Association Constant) in DNA - DNA hybridization.
2.How can i confirm about the DNA-DNA Hybridization by experiments.
Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...
04 May 2019 5,937 1 View
Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...
02 March 2019 2,987 2 View
Hi all, How one can calculate the LoD (Limit of detection) and LoB (Limit of Blank) for DNA based biosensor (Label Free). Should one has to take LoB after immobilizing the DNA probe...
31 December 2018 6,223 2 View
Hi all, What are the possibilities of a oxidized nucleobase, getting paired with the complementary base. does it satisfies Chargaff rule ? Can 8-Oxo-Guanine be paired with Cytosine ?
31 December 2018 948 4 View
Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...
11 December 2018 736 2 View
How do they fit the Randle Sevick's Equivalent circuit for multiple data (Nyquist Plot). Since most of the journals shows a single equivalent circuit representing for multiple nyquist plot .
08 September 2018 5,932 5 View
Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...
05 June 2018 5,656 3 View
Hi, Most of the Institutes and Research centers has skilled staff who may be just matriculation passed, as they get experienced they would tackle any troubleshoot or problems they face. Most...
05 June 2018 5,096 3 View
Hi, Is it possible to quantify the amount of DNA that has been immobilized on the gold electrode ?. How to quantify using EIS/ Chronocoulometry or any other methods.
04 May 2018 9,253 3 View
Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...
03 April 2018 1,708 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View