6-Mercaptohexanol in DNA biosensor ?

Hi, In most of the DNA biosensor experiments and articles they have used MCH (6-Mercaptohexanol) as a blocking agent, to orient DNA 90o and to remove non-specifically bound from the...

04 May 2019 5,748 1 View

How would i fix a DNA probe concentration for a biosensor ?

Hi all, Am working on a DNA based biosensor, DNA are thiolated. i reduce thiolated ssDNA by TCEP and dip cast it on a gold nanoparticle coated electrode. I have tried with 500nM ,...

02 March 2019 2,832 2 View

Hybridization of Oxidized nucleobase ?

Hi all, What are the possibilities of a oxidized nucleobase, getting paired with the complementary base. does it satisfies Chargaff rule ? Can 8-Oxo-Guanine be paired with Cytosine ?

31 December 2018 799 4 View

Electrochemical DNA Biosensor - LoD and LoB ?

Hi all, How one can calculate the LoD (Limit of detection) and LoB (Limit of Blank) for DNA based biosensor (Label Free). Should one has to take LoB after immobilizing the DNA probe...

31 December 2018 6,061 2 View

Oligonucleotides - NanoDrop Analysis ?

Hi All, How one can quantify the oligonucleotides for 10 - 100pM range, i had used nanodrop to quantify but ended up with the result same as blank. The oligo's had dispensed in...

11 December 2018 593 2 View

Electrochemical Impedance Spectroscopy ?

How do they fit the Randle Sevick's Equivalent circuit for multiple data (Nyquist Plot). Since most of the journals shows a single equivalent circuit representing for multiple nyquist plot .

08 September 2018 5,756 5 View

No X and Y- axis values in CH electrochemical Workstation graphs ?

Hi, Am using Electrochemical workstation 660E, Am not able to get X and Y axis values, have tried from rebooting the system to deleting the regedit includes deleting old data. if someone faced...

05 June 2018 5,523 3 View

Authorship for a Research Work ?

Hi, Most of the Institutes and Research centers has skilled staff who may be just matriculation passed, as they get experienced they would tackle any troubleshoot or problems they face. Most...

05 June 2018 4,952 3 View

How to quantify DNA immobilized on Electrode ?

Hi, Is it possible to quantify the amount of DNA that has been immobilized on the gold electrode ?. How to quantify using EIS/ Chronocoulometry or any other methods.

04 May 2018 9,097 3 View

Gold Nanoparticles Coated on Gold Electrode?

Hi, How difficulty is to remove the AuNp's coated on to the Gold electrode surface, can it be removed completely 100% ? Can one remove the AuNp's by Polishing with Alumina Powder and...

03 April 2018 1,474 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View