petri plate ,Tube , the explant was inoculated MS medium and kept 2 days for dark after exposure to light ,water vapour formed in the top of the plate and tube. How can we prevent this?
Do you know about transpiration, this is vital function, as long as plant material is there certainly you see water droplets, in presence of light stomatal opening is active, by the way what is your problem if you have water vapor?
Hi,
Ramadevi is correct, check humidity and temperature in your culture room,It may be a reason
@ Jetty Ramadevi, there is no need to be rude, if you consider a question to be silly, just ignore it. I think the question is so valid that research and engineering are applied to solve this issue.
Rakkimuthu, if using shelves, it is important to avoid direct contact of the upper shelf with the lamps underneath, and have a good air flow for even heat distribution. You can check some of the features that commercial growth chambers use to minimize this problem: http://www.percival-scientific.com/products/plant-tissue-culture
Alternatively, you could try this approach, provided it doesn't affect the growth of your plants: http://www.srs.fs.usda.gov/pubs/rn/rn_so369.pdf
Hi! Gustav, is it appearing that I am rude. May be truth is always bitter. Even you have droplets in tissue culture tubes it is not that serious problem as per my experience so I mentioned what is the problem if you have droplets, I worked a lot on banana. Hence mentioned last "do not think otherwise" by giving example.
Cheers!!!
Dont bother about water droplets. Just subculture it after 7 days. I think no problem will occur.
Thanks.
Some time these droplets became serious source of spreading bacteria in the Petri plates and test tubes, so his (Rakkimuthu) concern is quit valid. If some body want to avoid these droplets, we should try to solve his problem. Anyway as Gastavo said one has to check the air and temperature distribution in the place where these Petri plates and test tubes were kept. If one can ensure the equal distribution of temperature, then surely there will be no or less water droplet. The major reason for these droplets is the evaporation of water from agar and condensation in the lid or tube walls (having low temperature). So, I suggest using one-two days old plates or tubes, place an insulator (like paper towel) below petri plate and can wrap your test tubes with paper to maintain constant temperature. I hope this will work for you...:-)
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