Can we use two antibodies generated in same species for indirect Sandwich ELISA?

I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...

11 January 2021 1,754 4 View

Is there any selective media to grow filamentous fungus only (not with yeast)?

22 November 2020 5,370 7 View

Do anyone know a NK cell line expressing FC receptor to perform ADCC assay?

Hello All, I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell...

16 November 2020 4,472 3 View

How to coat plasma sample (Antigen) in ELISA plate for a direct ELISA?

I have generated an antibody against an antigen. Now, I want to use the developed antibody in the detection of the unknown sample. So, I want to coat the plasma sample as antigen. How that can be...

26 October 2020 2,259 7 View

I want to simulate a VAR model. How do I choose the coefficients to ensure stability?

I know how to compute eigenvalues to test stability. The question If i have for example a 10 node model with 5 lags, how do i choose stable parameters without just guessing.

06 October 2020 5,238 6 View

How Big data Analytics used in Crop yield prediction?

How Big data Analytics used in Crop yield prediction?

23 September 2020 8,653 4 View

Which path loss model will be suitable considering the scenario of factory automation with 5G URLLC?

In the performance analysis of modern wireless communication systems like the scenario of factory automation with 5G URLLC, which path loss model will be suitable and appropriate to assume? Any...

03 July 2020 918 2 View

How can i ensure the complete oxidation of graphite before quenching the oxidation process by H2O2?

I am following KMnO4 -H2SO4 reaction media for the synthesis of graphene oxide(GO) by an improved process.

04 June 2020 508 3 View

What is the pebbling number of a cycle graph with 4 vertices?

i need the number of pebbles for a cycle graph with 4 vertices

24 September 2019 4,188 1 View

Anyone know where the micro tensile testing machine available?

In Chennai where micro tensile testing machine available

19 August 2019 3,926 2 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Any reference paper with detailing on how to hybradize Q-Learning algorithm with Genetic Algorithm, especially for a variable optimization problem?

I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...

07 February 2021 3,123 8 View

How to compare expected and counted values statistically with different p values for each genotype?

Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...

03 February 2021 6,408 2 View

Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

Are Signal Peptide and Transit Peptide different? And what are their functions?

I want to learn about mutation in transit peptide. Can mutation in transit peptide affect the function of the protein?

20 January 2021 396 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

How Does a Mutation Undercut The Human Immune System to the COVID-19 Virus?

A handful of mutations to SARS-CoV-2 can help it to escape the immune response mounted by a subset of infected people. https://www.nature.com/articles/d41586-020-00502-w Comprehensive mapping of...

13 January 2021 9,545 3 View

Variant or strain, what exactly happened to the corona virus ?

The new, more contagious variant of the virus that causes COVID-19 which initially detected in the U.K. and has now been spotted in many of the country. While some have referred to this as a new...

11 January 2021 8,810 5 View

Journals with low APC or no APC?

Do you know any journals in the area of medicine/pediatrics/nutrition that publish protocols of systematic reviews? No APC or Low APC

04 January 2021 292 14 View