Nikhil Job

6 Questions 5 Answers 0 Followers

Questions related from Nikhil Job

How can I isolate RNA from Arabidopsis dry seeds?

Can anyone suggest me a better protocol for extraction of total RNA from Arabidopsis dry seeds? I tried with commercially available kit for extraction but the yield was very less. I used around...

01 January 2016 6,705 6 View

How to remove contamination from Arabidopsis seedlings?

My Arabidopsis plants growing on MS media got contaminated by both fungus and bacteria after the transfer to fresh media. Please suggest some method to remove theses contamination. Thank you

21 August 2014 1,674 9 View

How can I separate cells from callus?

I would like to separate individual cells from my Orchid callus. What is the best way to do this, without damaging the cells?

07 September 2013 287 12 View

Are synthesized nanoparticles permeable through animal cell membranes under normal physiological conditions?

What is the the size range and chemical nature of nanoparticles which can pass through cell membranes? If it is not permeable how can we induce its permeability?

02 September 2013 9,437 6 View

Any physical treatment for TCL explants?

My TCL explants(orchid family) are not responding to MS medium with different combination of PGRs. Can any physical treatment give result?

14 July 2013 8,262 0 View

Does anyone have experience with non-responsive plants?

I am culturing some orchid explants (TCL) in MS medium with PGRs for 40 days, but the plants are neither responding or dying, a yellow color is replacing the green color of explants.

11 July 2013 5,702 4 View