Nikhil Job

12 Questions 8 Answers 0 Followers

Questions related from Nikhil Job

How can I isolate RNA from Arabidopsis dry seeds?

Can anyone suggest me a better protocol for extraction of total RNA from Arabidopsis dry seeds? I tried with commercially available kit for extraction but the yield was very less. I used around...

01 January 2016 6,708 6 View

Can anyone provide a simple biochemical protocol for quantification of lipid content in seeds ?

I want to measure the total lipid content from Arabidopsis seeds. Please suggest me a simple biochemical assay protocol for the quantification of lipids from dry seeds. Is there any reliable...

01 January 2016 1,783 3 View

Which commercially available competent cells (DH5a) work good for larger vectors after the LR reaction?

I am doing LR reactions of my insert (about 2 kb in a donor vector) with my destination vector of size 17 kb, but I getting very colonies. I think my competent cells are not that efficient to take...

19 February 2015 3,219 1 View

How to remove contamination from Arabidopsis seedlings?

My Arabidopsis plants growing on MS media got contaminated by both fungus and bacteria after the transfer to fresh media. Please suggest some method to remove theses contamination. Thank you

21 August 2014 1,678 9 View

How can I separate cells from callus?

I would like to separate individual cells from my Orchid callus. What is the best way to do this, without damaging the cells?

07 September 2013 291 12 View

Are synthesized nanoparticles permeable through animal cell membranes under normal physiological conditions?

What is the the size range and chemical nature of nanoparticles which can pass through cell membranes? If it is not permeable how can we induce its permeability?

02 September 2013 9,440 6 View

Clonality of stem cells?

What are the experimental ways to study or prove clonality of stem cells?

01 September 2013 6,373 5 View

Can anyone advise on why contamination occurs in a plant tissue culture tubes after 40 days?

How to eliminate this problem ? I am using MS medium with PGR ,and working with orchid's

22 July 2013 8,813 5 View

Any physical treatment for TCL explants?

My TCL explants(orchid family) are not responding to MS medium with different combination of PGRs. Can any physical treatment give result?

14 July 2013 8,264 0 View

Can anyone suggest the best way to sterilize coconut water using the MS medium for plant tissue culture?

I filter sterilize the coconut , boiled and added directly to the MS medium,then autoclaved it in 121(15 min).but lots of contamination is coming in the medium itself.

13 July 2013 8,957 6 View

Does anyone have experience with non-responsive plants?

I am culturing some orchid explants (TCL) in MS medium with PGRs for 40 days, but the plants are neither responding or dying, a yellow color is replacing the green color of explants.

11 July 2013 5,706 4 View

Which wavelength is suitable for plant regeneration from explant of orchidaceae?

Which wavelength will produce responses in plant tissue culture?

01 July 2013 9,806 1 View