I need to prepare 1000microg/ml and I could not figure out the amount of glucose which I have to weight.
It's the same as 1mg/ml. So if you need e.g. 100ml, add 100mg. For small volumes, prepare a 10x stock solution and dilute appropriately.
As mentioned above, it is 1 mg/ml or you could see it as 1g/L.
just prepare a stock solution at 1g/l, ie 1mg/ml and dilute it 3 times by 10...
fred
@Faiza
It depends on how much you need...it is 1 mg/ml, so to prepare one liter you have to weight I g and complete to one litre by your solvent
Frederic Lepretre the final solution wanted is 1mg/ml, so the stock shouldn't be diluted. It just depends on the volume needed, and what's easier to weigh (I prefer weighing 1 g over 1 mg personally)
As mentioned above, this concentration equal to 1g/L. We can pour 1 g glucose in a jojeh balloon (1L) and then fill the ballon with water to 1 L.
I need to check protein expression via SDS, therefore I wanted to know the exact procedure of IPTG induction. whether I give induction in an overnight grown culture or should I go for secondary...
23 April 2024 440 3 View
Novice in Western Blotting, First time I did I got these blue Streak after CBB staining and De-staining , what Could be the issue ???
27 February 2024 7,355 0 View
Hello. Have you ever observed calcofluor white causing a color change to purple-pink in colonies of Candida albicans?
30 January 2024 2,216 1 View
Information about, how to download and use them for identification of fungus is needed.
28 November 2023 7,739 1 View
Crispr cas9, off-target effects
10 October 2023 668 0 View
In 'life cycle' under product stages we can give 'waste disposal' process,how does this impact the result? And under product stage there is also option to make 'waste treatment' stage.So how to...
26 September 2023 4,261 1 View
Hello, I am facing an issue with my polyacrylamide gel for western blot. This is 12% gel and I have used precast gel and made it with the recipe provided by BIO-RAD. I have been using the same...
14 August 2023 158 3 View
DOI https://doi.org/10.1039/D1QI00869B , here slow evaporation method was used. But here ChemInform Abstract: Hydrothermal Synthesis, Nonlinear Optic... they used hydrothermal method. In another...
02 August 2023 9,399 0 View
I have been trying to culture cells pancreatic cancer cells and normal cells (GM05757). fIRST CELLS DONOT ATTACH TO THE FLASK EVEN FEW CELL ATTACHED TO THE FLASK BUT THEY DONOT DIVIDE AND AFTER...
27 July 2023 9,948 2 View
Please , I m working about fish nutrition but I had these symptoms, can you help me to identify pathology? Is it bacterial causes ? I think it's because the fight between males. Fish is tilapia...
25 July 2023 4,965 6 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
how i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water
08 July 2024 9,104 2 View
I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...
26 June 2024 3,664 1 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View
I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...
12 June 2024 7,331 1 View
Hi all, I have been attempting to do field EPSP but I am not able to elicit a response. I am using a thin walled electrode for the recording electrode at 1.5mOhm resistance. I have filled the...
10 June 2024 2,418 0 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View
Hi, we're trying to determine two amiglucosides antibiotics and one aminoacid in a powder mix whose principal ingredient is glucose: 60 % w/w. The antibiotics are amikazine and apramycin, the...
22 May 2024 5,636 0 View
After lapse of a few months, we recently attempted insulin-tolerance tests in C57BL/6N female mice at 6 months of age. We began, years ago, with Humulin-r at 0.75 U/kg. In our recent tests we...
22 May 2024 3,797 3 View