31 January 2024 1 2K Report

Hello. Have you ever observed calcofluor white causing a color change to purple-pink in colonies of Candida albicans?

More Faiza Tebbji's questions See All
For IPTG induction, should I use overnight culture or secondary culture and after how many hours of IPTG induction, we should take samples for SDS?

I need to check protein expression via SDS, therefore I wanted to know the exact procedure of IPTG induction. whether I give induction in an overnight grown culture or should I go for secondary...

23 April 2024 440 3 View

Can Someone tell me what could go wrong in SDS PAGE where I am getting there Blue dotted blots after CBB staining ???

Novice in Western Blotting, First time I did I got these blue Streak after CBB staining and De-staining , what Could be the issue ???

27 February 2024 7,353 0 View

I am looking for any book, taxonomic guides, or database for identification of fungus isolated from fruits. Please guide me?

Information about, how to download and use them for identification of fungus is needed.

28 November 2023 7,738 1 View

What are predictive models in crispr cas9?

Crispr cas9, off-target effects

10 October 2023 668 0 View

What is the difference between waste/disposal scenario and waste treatment in SimaPro?what is the use of them individually?

In 'life cycle' under product stages we can give 'waste disposal' process,how does this impact the result? And under product stage there is also option to make 'waste treatment' stage.So how to...

26 September 2023 4,261 1 View

I am facing an issue with the polyacrylamide gel for western blot. What could be the reason?

Hello, I am facing an issue with my polyacrylamide gel for western blot. This is 12% gel and I have used precast gel and made it with the recipe provided by BIO-RAD. I have been using the same...

14 August 2023 157 3 View

Why sometimes slow evaporation method is applied in order to synthesis noncentrosymmetric compounds?

DOI https://doi.org/10.1039/D1QI00869B , here slow evaporation method was used. But here ChemInform Abstract: Hydrothermal Synthesis, Nonlinear Optic... they used hydrothermal method. In another...

02 August 2023 9,398 0 View

Can someone please tell me what,s this in my pancreatic cells flask?

I have been trying to culture cells pancreatic cancer cells and normal cells (GM05757). fIRST CELLS DONOT ATTACH TO THE FLASK EVEN FEW CELL ATTACHED TO THE FLASK BUT THEY DONOT DIVIDE AND AFTER...

27 July 2023 9,947 2 View

Can you help me to identify this symptoms of tilapia?

Please , I m working about fish nutrition but I had these symptoms, can you help me to identify pathology? Is it bacterial causes ? I think it's because the fight between males. Fish is tilapia...

25 July 2023 4,964 6 View

Recombinant protein purification from prokaryotic expression system centrifugation at 16000rpm for 16 min can be replaced by 4000rpm for 60min?

By keeping rest of the protocol same does centrifugation speed will have any effect on protein extraction from XL1-blue colonies

26 June 2023 2,311 3 View

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Do we save MSMEs of the Nation ?

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What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 192 4 View

Why applying a saved template of a bar chart in a different/new one doesn't take effect in SPSS?

Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...

29 July 2024 1,980 1 View

In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...

28 July 2024 6,514 1 View

Dear colleagues, Can anybody by chance have pdfs of two books in Chinese: Liu (2003) and Zhang et al. (2004)?

Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression，Tarim Basin of Xinjiang，NW China. Palaeontologia Sinica， New Series A，no. 14（Whole no. 190）....

28 July 2024 6,390 1 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 284 0 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,132 0 View

What is the best way to drop a plasmid?

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25 July 2024 2,532 3 View

Aqua regia not heating up?

I used Aqua regia to clean Au nanoparticles in the used glassware. But for some reasons, it is not heating up upon mixing. The color did change from no color to light orange. The Au NPs on the...

25 July 2024 4,387 0 View

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