I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and cofactors to produce a red color (500 nm) corresponding to the glucose concentration. I would like to mimic the conditions in milk without actually using milk (it's not transparent!). What should I add and how much? Ca? Mg? etc.?
Nick Chacos
First of all, assays on samples like milk are usually precede by treatment of the sample with a Carrez reagent which clarifies the sample by removal of protein and/or lipids. Once that is accomplished, the assay is straightforward.
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