Novice in Western Blotting, First time I did I got these blue Streak after CBB staining and De-staining , what Could be the issue ???
I need to check protein expression via SDS, therefore I wanted to know the exact procedure of IPTG induction. whether I give induction in an overnight grown culture or should I go for secondary...
23 April 2024 440 3 View
Hello. Have you ever observed calcofluor white causing a color change to purple-pink in colonies of Candida albicans?
30 January 2024 2,216 1 View
Information about, how to download and use them for identification of fungus is needed.
28 November 2023 7,739 1 View
Crispr cas9, off-target effects
10 October 2023 668 0 View
In 'life cycle' under product stages we can give 'waste disposal' process,how does this impact the result? And under product stage there is also option to make 'waste treatment' stage.So how to...
26 September 2023 4,261 1 View
Hello, I am facing an issue with my polyacrylamide gel for western blot. This is 12% gel and I have used precast gel and made it with the recipe provided by BIO-RAD. I have been using the same...
14 August 2023 157 3 View
DOI https://doi.org/10.1039/D1QI00869B , here slow evaporation method was used. But here ChemInform Abstract: Hydrothermal Synthesis, Nonlinear Optic... they used hydrothermal method. In another...
02 August 2023 9,399 0 View
I have been trying to culture cells pancreatic cancer cells and normal cells (GM05757). fIRST CELLS DONOT ATTACH TO THE FLASK EVEN FEW CELL ATTACHED TO THE FLASK BUT THEY DONOT DIVIDE AND AFTER...
27 July 2023 9,948 2 View
Please , I m working about fish nutrition but I had these symptoms, can you help me to identify pathology? Is it bacterial causes ? I think it's because the fight between males. Fish is tilapia...
25 July 2023 4,964 6 View
By keeping rest of the protocol same does centrifugation speed will have any effect on protein extraction from XL1-blue colonies
26 June 2023 2,312 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,323 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,164 1 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,686 3 View