Crispr cas9, off-target effects
I need to check protein expression via SDS, therefore I wanted to know the exact procedure of IPTG induction. whether I give induction in an overnight grown culture or should I go for secondary...
23 April 2024 440 3 View
Novice in Western Blotting, First time I did I got these blue Streak after CBB staining and De-staining , what Could be the issue ???
27 February 2024 7,355 0 View
Hello. Have you ever observed calcofluor white causing a color change to purple-pink in colonies of Candida albicans?
30 January 2024 2,216 1 View
Information about, how to download and use them for identification of fungus is needed.
28 November 2023 7,739 1 View
In 'life cycle' under product stages we can give 'waste disposal' process,how does this impact the result? And under product stage there is also option to make 'waste treatment' stage.So how to...
26 September 2023 4,261 1 View
Hello, I am facing an issue with my polyacrylamide gel for western blot. This is 12% gel and I have used precast gel and made it with the recipe provided by BIO-RAD. I have been using the same...
14 August 2023 158 3 View
DOI https://doi.org/10.1039/D1QI00869B , here slow evaporation method was used. But here ChemInform Abstract: Hydrothermal Synthesis, Nonlinear Optic... they used hydrothermal method. In another...
02 August 2023 9,399 0 View
I have been trying to culture cells pancreatic cancer cells and normal cells (GM05757). fIRST CELLS DONOT ATTACH TO THE FLASK EVEN FEW CELL ATTACHED TO THE FLASK BUT THEY DONOT DIVIDE AND AFTER...
27 July 2023 9,948 2 View
Please , I m working about fish nutrition but I had these symptoms, can you help me to identify pathology? Is it bacterial causes ? I think it's because the fight between males. Fish is tilapia...
25 July 2023 4,965 6 View
By keeping rest of the protocol same does centrifugation speed will have any effect on protein extraction from XL1-blue colonies
26 June 2023 2,312 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...
05 August 2024 3,665 0 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...
31 July 2024 9,733 3 View
