How to prepare 0.035M phosphate buffer pH:4.5?

Burcu Uner @Burcu-Uner

19 November 2020 1 4K Report

I want to prepare mobile phase has to be adjusted to pH 4.5 with o.035M phosphate buffer

Can anyone help me to prepare phosphate buffer solution of 0,035M using pH of 4.5 ?

waiting for your favorable response,

thanks

Adeyemi Oluwadare Ayodeji

Burcu Uner Though, I don't know what exactly you are aiming at, I think it would be better to use a sodium citrate buffer or an sodium acetate buffer at pH 4.5 because the buffering capacity of Phosphate buffer is best between pH 6 and 7.5

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