How can i remove the error bar from the legend?

xdata1=tempmeasS2.Timesec; ydata1=tempmeasS2.UCNP; plot (xdata1,ydata1) err2 = tempmeasS2.UCNP1; e2=errorbar(xdata1,ydata1,err2, 'b-', 'lineWidth', 1, 'HandleVisibility', 'on') legend ('1...

13 January 2021 3,140 3 View

How to prepare 0.03M phosphate buffer pH:4.0?

I want to prepare mobile phase has to be adjusted to pH 4 with o.03M phosphate buffer Can anyone help me to prepare phosphate buffer solution of 0,03M using pH of 4 ? waiting for your favorable...

18 November 2020 210 3 View

What is the most reliable method for starch digestibility analysis in beans?

I found papers cited Englyst et al. (1992) and mentioned Megazyme kit, But unfortunately these were not clear and hard to follow. Thanks in advance,

23 September 2020 1,476 5 View

What is the reason of spongy structure of nanocellulose after freeze drying step?

To obtain dried powder nanocellulose, we used freeze drying method but the result is nanocellulose sponge not powder. Could we have failed because the acid was not sufficiently removed from the...

05 January 2020 6,277 2 View

Have you ever used OCAI organizational culture assessment tool?

I would like to use Competing Values Framework organizational culture assessment tool OCAI for my research and need some advice about its Likert version. I would be glad to hear the experiences of...

14 November 2017 3,467 2 View

Why do we calculate negative encapsulation efficiency in plga nanoparticle?

Hello, I need to help about my thesis. I syhnthesize catechin loaded plga nanoparticle. I use indirect analysis method for calculate encapsulation efficiency and I get negative encapsulation...

06 November 2016 2,464 4 View

Why my agarose gels drop off in neutralization buffer during alkaline comet assay?

21 June 2016 839 12 View

What is the normal range of fasting serum insulin in rats?

What is the normal range of fasting serum insulin in rats? I could'nt find any range mlU/L?

04 June 2016 9,616 9 View

Is there anyone to merge DAPI-Tunel images?

27 January 2016 7,240 2 View

Has anybody experienced having false positive results in a calcium flux assay by flow cytometry?

False positive results in calcium flux assay

28 August 2012 697 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View