I am working with PEN membrane slides used in laser capture microdissection. When I try to cut the cells on the membrane, too many bubbles are seen. I made a tiny hole on the edge of the membrane and water came out. I did dehydratation steps after IHC and put the slides 60 C inside the hot plate to make them dry but I couldn't get rid of water without making a hole on the membrane. Do you have any idea what could be the reason of the water or any suggestions to make water-free slides? TIA