Hello,

Human fibroblast cells are cultured in DMEM +10%FBS.

For your experiment you can use DMEM with low glucose and sodium pyruvate, but without phenol red and L-glutamine.

This medium is available at Thermo Fisher Scientific (Gibco)

Cat# 11054001.

Best.

thank you for your answer. So Is there any reason not to use l glutamine?

You can supplement the media with 2mM L-glutamine if required.