The procedure compute?

02 March 2021 5,204 3 View

One-sided testing of MLM in R?

Dear colleagues, Does anyone know how to conduct a one-sided testing of regression weights (MLM) in R? The actual model is fm1rs

23 January 2021 3,970 3 View

[Measurement invariance] - Do you test it on the full SEM model, on each construct separately, or both?

Hi everyone, I would like to know whether both cultural groups interpreted my questionnaire in a similar way. To do so, I conducted a measurement invariance analysis on the whole model of latent...

14 January 2021 1,845 3 View

Is it possible to do a closed loop control of a sine wave?

An analogue voltage sine wave signal is send from a PLC to a Piezo actuator. This actuator converts the voltage to force. A load cell checks what the applied force is and sends a mA-signal...

27 December 2020 7,037 3 View

[SEM] Why RStudio and AMOS report different p-values (but same estimates)?

Hi everyone, I have conducted a SEM analysis in AMOS then replicated it in RStudio. While I obtained identical estimates and model fit statistics, the p-values differ between software programs...

25 December 2020 3,669 4 View

What should my boundary conditions be in abaqus and how do I apply a sine load?

I want to model a T bone specimen that is subjected to a sine load. The specimen is clamped in as showed on the figure. I was wondering what I should chose as boundary conditions and how I can...

08 November 2020 9,766 4 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Is there any possibility to get the estimated values (listed for every participant) of a specific latent variable in R or Mplus?

Is there any possibility to get the estimated values (listed for every participant) of a specific latent variable in R or Mplus?

17 June 2020 3,819 3 View

Literature for multilevel MCMC models?

Hey there, I am looking for literature or tutorial videos, which are concerned with the question of how multilevel markov chain monte carlo models are conducted in R or Mplus. Can someone help...

20 April 2020 7,801 1 View

How to generate a ROC-Curve for multilevel regression models?

Dear colleagues, Does anyone know, how to build a ROC-Curve and a AUC-Value concerning multilevel logistic regression models using R or Mplus. I would be very grateful for any articles,...

26 February 2020 8,930 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View