Hi,

I want to make a 10% SDS-PAGE gel that contains 6M urea. Both the resolving and stacking buffers should have urea. Could anybody please suggest how to do this? I prepared my samples in buffer containing Tris-Cl, SDS, glycerol and bromophenol blue followed by heating at 100C.

I want to make a 10% resolving gel and 5% stacking gel both containing 6M urea.

Please advise.

Thanks

Sumit

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