How to make urea-sds-page gel?

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How to make urea-sds-page gel?

Hi, I want to make a 10% SDS-PAGE gel in which both the resolving and the stacking would contain 6M urea. How would I prepare them? I have already prepared my samples in buffer containing...

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Anand Patwardhan

Hi,

Yes, its regular Acrylamide % gel, just add urea as per your concentration. Urea should be added in both stacking & resolving. I remember running such gel in cold room to avoid heating.

Maintain sterile RNAase free conditions & water.

Good luck,

Anand

Raju Ahmed

This link might be helpful for you. https://www.nature.com/nprot/journal/v1/n1/pdf/nprot.2006.4.pdf