How to isolate Neutrophils for the study of NETosis ?

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Malcolm Nobre

Hello Aleena Varughese

Please refer to the article attached below. It provides both direct and indirect methods of evaluating the NETosis process.

https://www.jpccr.eu/pdf-105563-51261?filename=Direct%20and%20indirect.pdf

Best.

Aleena Varughese

Hello Malcom

thank you for your suggestion

Nuruddeen Lewis

I'm not sure if you are just looking for a method to isolate neutrophils or methods to assess NETosis; I think the above answer gave a lot of options to measure NETosis. The link I'm providing here is a good way to isolate human neutrophils: Article Neutrophil Isolation Protocol