Which one should be the control point and slave node in assigning MPC constraint using ABAQUS?

Hello everyone, I am modeling a bridge with and orthotropic deck, floor beams, edge beams and main girders. I have to connect bridge deck with the floor beams and floor beams with the main...

22 November 2020 5,750 4 View

What is the best suited element type for meshing the notched parts of structural steel members?

Hello every one, I am doing finite element analysis of a notched steel component with nonlinear material properties. I have tried different element types for meshing and the analysis results are...

11 October 2020 5,433 3 View

How the optical property will modify based on penn energy?

For single crystal

18 September 2017 1,650 1 View

Can you send a code for tweet segmentation?

12 April 2017 8,236 2 View

How do we exactly analyze ELISA results obtained from ELISA reader and draw conclusions from it?

Calculation of results from ELISA data

04 June 2012 1,825 4 View

How can I improve RNA extration from human neutrophils?

I tried several times extrating RNA from human neutrophils and the amount of RNA at the end of the precedure was minimal. I also tried using colunns from QIAGEN. Never had any problem with tissue...

26 January 2021 8,881 4 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

25 November 2020 2,680 3 View

Dissociation of stimulated neutrophils for flow cytometry?

I realize that the work flow in most flow-based studies of neutrophils is such that the cells are analyzed without ever becoming adherent to the cell culture surface, ie, as soon after isolation...

14 October 2020 1,579 4 View

Is Sytox (from Thermo Fisher Scientific) a good choice to dectect NETs in flow cytometry using whole blood?

I found several papers in the literature where Sytox (green, red, blue, or orange) is used to detect Neutrophil Extracellular Traps, but it's always used with purified neutrophils. Do you know if...

07 October 2020 5,865 2 View

Is this typical lymphocyte, the flower cells?

21 September 2020 4,107 3 View

Does LPS elicit an inflammatory response in the neutrophil-like cell line HL-60?

I'm replacing primary neutrophils with the neutrophil-like cell line HL-60 for immunological studies. Do HL-60 cells, differentiated towards the granulocyte phenotype with retinoic acid or DMSO,...

07 June 2020 2,219 3 View

Sample size determination ?

I am going to study about cell population data generated by automated hematology analyzers related to Neutrophils in sepsis.Study will compare medians of several parameters between normal control...

29 May 2020 5,554 3 View

Correct use of Grubbs-test: outlier for one parameter = outlier for all parameters?

Hi everyone, let me clarify this with an example: Lets say, I have an animal model with two read-out parameters, like for example neutrophil and monocyte infiltration. If mouse A is identified as...

29 March 2020 9,693 3 View

Can I use LS column and MidiMACS seperator from Miltenyi Biotec Neutrophil Isolation Kit for mouse peripheral blood?

We are using the neutrophil isolation kit from Miltenyi Biotec to get the neutrophils from peripheral blood from JAK2 constitutively active mice. This mouse is a leukemia model, so the traditional...

11 March 2020 8,611 3 View

Specific marker for neutrophil?

Hi, I will be carrying out flow cytometry using whole blood (human) and I'm looking for a marker that is specific to human neutrophils only. Any suggestions? Thank you.

08 March 2020 9,612 3 View