How to count neutrophil after isolation from whole blood from human by density gradient centrifugation using GSM1119 and LSM1077?

More Rambha Jha's questions See All

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Does behavior (shy vs bold; rare vs abundant) and flock size (small vs large) of bird species play role in niche partitioning among sympatric species?

Sympatric birds may avoid competition via multiple strategies - by different activity period, different diet, different microhabitat etc. Has any one looked into behavior of sympatric birds as...

11 January 2021 9,600 1 View

Which type of polymer is used in base boards of Printed Circuit Boards (PCBs)?

I want to know about the type of polymers in PCBs.

25 May 2020 9,640 15 View

Thermal survey equipment in Hyderabad area ?

I am looking to hire thermal survey equipment for study of coal stockpiles in thermal power plants in Hyderabad area. If you have any leads please let me know. thanks

24 February 2020 8,702 3 View

Dear Peers! Could you please oblige me with your knowledge on vulture?

I am looking for proverbs and idioms or any short story mentioning vulture in any language or locality with English translation.

24 February 2020 2,004 9 View

I am supposed to develop a psychometric employability assessment. Any literature or advice that could help me? Also, how do I ensure honest answers?

I have never done this before. So far I have collected scales for different factors after detailed research. I plan on taking items from them and maybe restructuring them to make a proper...

31 January 2020 9,923 2 View

Which funding agency in India will grant traveling allowance for international conference in finance ??

I get an opportunity to present my paper in international conference on June 2020. I am research scholar in management (finance department).Kindly let me know which funding agency will grant...

07 January 2020 7,040 1 View

Can anyone suggest me a solver for binary linear multiplicative programming problem?

The example case is as follows: Maximize(a1 * X1 + a2 * X2 + a3 * X3 + a1 * a2 * Y12 + a1 * a3 * Y13 + a2 * a3 * Y23) constraints are: a1, a2, a3 are binary variable belongs to [0, 1], and a1 + a2...

23 December 2019 5,912 4 View

How can we estimate Nitrogen (N) in sugarcane field using satellite imagery?

I want to estimate nitrogen (N) content in the sugarcane fields using sentinel-2 optical data (Multi spectral Imagery). I have read few papers where simulated values helped in potatoes and other...

16 May 2019 4,580 6 View

How can we download the all the clean drug like compounds from Zinc12 database?

http://zinc.docking.org/subsets/drug-like When I click on sdf/mol2 format , it gives me usual.sdf.bat or usual.mol2.bat. How I will use these file to retreive the compounds????

08 April 2019 2,042 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

Antonio La Gioia

What do you use for counting? An automatic analyzer or a manual counting chamber (Burker type)?

Rambha Jha

I am using hemocytometer for counting the cell

Most cytometers use the impedance method for cell count.

Any change in the electrolyte state and distribution of electric charges in the cell suspension medium may interfere with the count.

Such could be the case after separation in density gradient. I recommend the washing of saline cells and their resuspension in compatible plasma or better in a mixture plasma-red blood cells (separated by centrifugation).

Let me know.

Alternatively and without additional manipulation the "old" Burker method could be, although time-consuming, resolutive