Hello!
I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell type?
Thanks in advance for any answers.
My fellow Academic colleagues! I together with my lab mates have a PCR-related issues that we hope that some(one) of you might have encountered and hopefully solved. In “short”, our initial PCR...
04 May 2024 8,788 5 View
When clicking on the publicatoin "Focal Cortex Stimulation With a Novel Implantable Device and Antiseizure Outcomes in 2 Prospective Multicenter Single-Arm Trials" by Schulze-Bonhage et al., the...
27 February 2024 5,522 0 View
Is there a direct relationship between the amount of galactomannan in the blood and the presence of Aspergillus in the lungs? In other words, does a higher level of galactomannan in the blood...
07 February 2024 9,725 0 View
Hello, do you think that full spectrum LEDs are less useful than red and blue light for growing green, leafy plants (e.g. lettuce, spiderwort, cress etc.)? I have read many articles on this and...
21 January 2024 6,736 3 View
The problem that I would like to solve is that two identical versions of a publication are listed as distinct RG-items in my account, because they have slightly different titles. Is it possible to...
08 January 2024 4,908 1 View
Certain of my book reviews are listed as my publications, In the edit section of the publications page, there is no category for "book review". and I don't want to get problems by claiming...
28 December 2023 3,545 1 View
Hello! My SY5Y cells shapes were normal when firstly received(Figure 1), but changed into something like amoeba after digesting with pancreatin/EDTA for merely 30s(Figure 2, red arrow). The cells...
14 November 2023 9,083 5 View
Dear colleagues, Unfortunately, I don't have MATLAB and I don't know anything about it. Could someone please help me? Given is the vector x0 = (1, 2, 3); What does the following code...
25 September 2023 9,363 10 View
Dear colleagues from the field of (Cognitive) Literary Studies, I conducted an empirical study on "Waiting for Godot" during my time as a graduate student and completed the initial manuscript of...
09 August 2023 8,911 5 View
Is it necessary for a charged particle to have a magnetic moment to be trapped in a magnetic bottle?
23 July 2023 9,480 8 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
01 July 2024 2,558 0 View
Hi, scholars. I am planning to conduct mouse neutrophil and T cell co-culture in Vitro and observe the effect of neutrophils on T cells. I don't have any protocol. Could anyone provide me...
19 June 2024 3,271 2 View
I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...
19 June 2024 4,210 3 View
What parameters are important in this process?
17 June 2024 4,463 0 View
i use zeta-view to detect exosome particles,
29 April 2024 9,318 2 View
I am currently preparing research methods for my thesis. My sample is an exosome from Adipose. However, I had difficulty identifying what types of proteins were in it. Can anyone help me?...
19 April 2024 4,524 2 View
As a clinical medicine student investigating the role of exosomes in acute kidney injury, my research has progressed to the point where I need to validate the contributions of exosomal membrane...
15 April 2024 8,330 0 View
15 April 2024 2,628 0 View