How to compute the refraction index for a 79.5GHz (3.8mm) FMCW Radar Instrument?

Dear everyone, I performed some experiements with a FMCW Radar Instrument emitting waves of 3.8mm. I can see some "movements" when I am looking on the relative displacements (+/- 0.15mm) over...

07 January 2021 2,073 6 View

Experience in using the LiDAR of the new iPad Pro for mapping?

Has anyone experience in using the LiDAR of the new iPad and if so, what App have you used? We want to map fault structures or rather fractures of damage zones and I wonder if this can easily be...

01 August 2020 7,473 4 View

Maleimide dye labelling of Ni2+ NTA resin?

Hi. When I purify my His-tagged membrane protein with IMAC, I label the protein (containing a single cysteine) with fluorescein-5-maleimide while the protein is immobilized to the Ni2+ NTA. I...

20 July 2020 2,065 3 View

Is anyone familiar with making a new cell line available for everyone? How to have ATCC or someone else to sell your cell line?

We have developed a new cell line and would like to make it available to everyone. Is anybody familiar with this process? What are the requirements? Are there other companies other than ATCC that...

19 May 2020 1,390 7 View

2-way ANOVA - splitting data or not?

Hi. My question is about 2-way ANOVA (and Freidmans test). I've no problem with the test per se but rather with the dataset in relation to the test(s). My problem is that I've 16 different...

06 April 2020 6,920 4 View

Why information criteria differ so i cant compare arma models?

Hello, i am trying to forecast using eviews the stock price returns of a specific company. I am running automatic arima forecasting so as eviews evaluates and proposes the best model fit...

30 March 2020 9,431 4 View

Paper for using PROCESS for mediation with two independent variables?

14 October 2019 356 3 View

How to stoichiometry calculate this combustion process?

Hi! This one is for you chemists/chemical engineers out there. I have a fuel, tall oil pitch, that I want to burn mixed with ordinary air in an industrial burner for heat generation. The tall...

01 October 2019 5,143 12 View

How to dissolve CaWO4?

Hi, What is the best way to dissolve pure CaWO4? I tried: - conc. Aqua Regia - conc. Aqua Regia + conc. HF - conc. HNO3 + conc. HF - 7N HNO3 - 7N HNO3 + conc HF. Every experiment was for 55...

29 September 2019 5,982 1 View

How can nanobuffering work?

Dear All I am wondering if someone has an good explanation for the "nanobuffering" behavior of pH responsive polymers. Here is some literature on the topic: Increasing Enzyme Cascade Throughput...

28 August 2019 5,254 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

How can I improve RNA extration from human neutrophils?

I tried several times extrating RNA from human neutrophils and the amount of RNA at the end of the precedure was minimal. I also tried using colunns from QIAGEN. Never had any problem with tissue...

26 January 2021 8,881 4 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

25 November 2020 2,680 3 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

What is the best RNA isolation method from exosomes of serum samples ?

12 November 2020 9,331 7 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

Could anyone recommend a kit to isolate exosome from cell culture supernatant?

Hello, I'm working with immortalized HUVECs low and I'm trying to study their exosomal RNA contents. Could anyone recommend an effective kit to isolate exosomes from cell culture supernatant?...

31 October 2020 4,252 5 View

How to extract extracellular vesicles from cell lines?

Recently, there was a problem with my qpcr results. I want to try more methods of extracting EV from the cell medium. At present, I am using Invitrogen's kit(Cat4478359) to extract exosomes. Can...

28 October 2020 2,961 3 View