how to make the disc of a particular nanoparticle concentration for antibacterial disc diffusion assay
Dear Aleena Haqqi
I have employed the disc diffusion method to determine antibacterial properties of iron oxide NPs and their core-shell structures and I have seen many other studies to do the same. Check out these refs:
https://www.sciencedirect.com/science/article/abs/pii/S014181301934351X
Article Ocimum basilicum mucilage as a new green polymer support for...
Better to go with disk diffusion method so you can incubate the plate in inverted position safely. Moreover, well diffusion assay is not standardized as the disk method.
Aleena Haqqi Greetings
According to studies, the disk diffusion method is a more appropriate method for determining the antibacterial properties of nanoparticles, but the type of microorganism studied in your research is important in choosing a more appropriate method to study the antimicrobial properties of nanoparticles,
Please take a look at the following links for more information:
Article Metal-Based Nanoparticles as Antimicrobial Agents: An Overview
Article Methods for in vitro evaluating antimicrobial activity: A review
Best wishes to you.
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