Why does my JC-1 probe form sediment when dissolved in RPMI?

04 December 2023 0 4K Report

Hi all,

I am attempting to use several fluorescent probes to evaluate various aspects of mitochondrial metabolism in neutrophils.

When I use my JC-1 probe it immediately clumps into sediment when I introduce it to my medium, and no amount of vortexing can dissolve these clumps.

For context, the probe is dissolved in DMSO at a 5mg/ml conc, and I dilute that into a 5ug/ml conc in complete RPMI media.

Any advice?

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