Hello Aleena Varughese
The recommended media is BEGM (Bronchial Epithelial Cell Growth Medium) which is specially designed to support the growth of human primary bronchial epithelial cells such as BEAS-2B. You may refer to the link below.
https://bioscience.lonza.com/lonza_bs/CH/en/Primary-and-Stem-Cells/p/000000000000185308/BEGM%E2%84%A2-Bronchial-Epithelial-Cell-Growth-Medium-BulletKit%E2%84%A2#:~:text=BEGMTM%20Bronchial%20Epithelial%20Cell%20Growth%20Medium%20is%20specially%20designed,from%20normal%20and%20diseased%20tissues.
You may also use LHC-9 media. Please see the link below.
https://www.thermofisher.com/order/catalog/product/12680013
You may use DMEM supplemented with 10% FBS. But I would not recommend this media because it is likely to change the cell phenotype which may be visible in culture after a week’s time.
Please refer to the below attached paper in which DMEM has been used to culture BEAS-2B cells.
Article Human bronchial epithelial BEAS-2B cells, an appropriate in ...
Best.
Yes, you can use DMEM media for culturing and maintaining BEAS-2B cells. DMEM (Dulbecco's Modified Eagle Medium) is a widely used medium for cell culture, and it supports the growth of many cell types, including BEAS-2B cells.
BEAS-2B cells are a type of bronchial epithelial cell line that is commonly used to study respiratory diseases and lung function. They are known to grow well in DMEM supplemented with fetal bovine serum (FBS) and other growth factors.
However, it's important to note that BEAS-2B cells require a specific set of conditions to grow optimally. Here are some tips for culturing and maintaining BEAS-2B cells in DMEM media:
1. Use a high concentration of FBS: BEAS-2B cells require a minimum concentration of 10% FBS in the medium to grow well. You can use up to 20% FBS for optimal growth.
2. Add penicillin-streptomycin: Penicillin-streptomycin is essential for preventing bacterial contamination in cell cultures. Add 100 IU/mL of penicillin and 100 μg/mL of streptomycin to the medium.
3. Maintain proper pH and temperature: Keep the pH of the medium between 7.2 and 7.4, and maintain the temperature at 37°C in a humidified incubator with 5% CO2.
4. Monitor cell density: Subconfluence cultures are recommended for optimal growth and maintenance of BEAS-2B cells. Seed the cells at a density of approximately 1-2 x 10^5 cells/cm² and split them when they reach confluency.
5. Passage cells regularly: To keep the cells healthy and prevent senescence, passage them every 3-4 days. Trypsinize the cells gently, and replenish the medium with fresh DMEM containing FBS and penicillin-streptomycin.
6. Consider adding additional supplements: Depending on your research requirements, you may want to add other supplements to the medium, such as hydrocortisone, cholera toxin, or retinoic acid, to support specific cellular processes or differentiation.
All the best
you can use BEGM kit or LHC-9 medium. Do not use DMEM for Beas2B cell-line.
- Badges
- Science topic
- Similar topics
- Instrumentation Engineering
- Instruments