Aleena Varughese

10 Questions 6 Answers 0 Followers

Questions related from Aleena Varughese

After running RT-PCR for beta actin i got curves after 20 cycles. is this right? Is it mandatory that the curves should come within 20 cycles?

what is the reason behind this?is it due to the cDNA containation? usually how much cycles we are considering for doing PCR ?

07 December 2023 1,135 0 View

How to help to trouble shoot Agarose gel elctrophoresis?

I ve run AGE for cDNA but i got like this.what is the reason behind this?

14 November 2023 170 5 View

For Beas 2b cell line can i use DMEM media for cell culture?

Is that ok to use DMEM media for maintaining beas2b cell line?

29 October 2023 5,832 3 View

Why beta actin used as a house keeping gene for RT-PCR and western blot ?

what is the normal range in case of cell lines?

29 October 2023 3,269 3 View

Which is the best way for cell lysis during RNA isolation sonication or mechanical vortexing?

Does cell lysis affect RNA isolation? I ve done my cell lysis by vortexing after that i ve done RT but im not geting the results.

29 October 2023 7,017 1 View

During my cell culture after revival the cells were getting stressed .What might be the reason behind it ?

How long we have to trypsinize A549 Cell as well as Beas2b cell line?? is it due to to fault during cryopreservation ?

29 October 2023 6,855 1 View

Why we use normal lung epithelial cell line or MCF cell line as a control for the study of lung fibrosis ?

As a control i thought to use A549 Cell line. which is an alveolar epithelial cell line.Is that possible to use another cell line like mcf or normal lung epithelium as control group? why ?

25 September 2023 6,121 2 View

How to isolate Neutrophils for the study of NETosis ?

Method to identify NETosis

28 June 2023 6,502 3 View

Can anyone describe about PCR primer designing ?

How to select appropriate primers in blast? what are the important things to keep in mind while selecting primers?

01 January 1970 2,746 5 View

Can anyone suggest protocol for protein separation using Biotynylated Streptavidin

How to separate protein using Biotynylated Streptavidin method? Is there any alternative procedure?

01 January 1970 1,318 0 View