Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Pseudo random number generator in Matlab code ?

i want to implement pseudo random number generator in matlab ...i request to every one if someone have code these PRNG share with me SplitMix Permuted Congruential Generator (PCG) Random...

14 February 2021 6,828 7 View

Please ,How can I correct this kind of error?

27 January 2021 4,815 5 View

How can I calculate convective heat transfer coefficient from turbulent boundary layer properties?

I know many textbooks include empirical relations between Nu, Re_x, and Pr inside a turbulent boundary layer on a flat wall. However, I want to directly calculate h from known boundary layer...

19 January 2021 1,294 3 View

What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View