Fatima Hassan

5 Questions 3 Answers 0 Followers

Questions related from Fatima Hassan

What are the reasons for unsuccessful ligation in a pJET1.2 cloning vector?

I am trying to sequence alleles by ligating short PCR products into a pJET 1.2 vector. Although pJET protocol states that the plasmid contains suicidal genes that are disrupted once ligation is...

31 January 2022 5,824 1 View

What is a good image analysis software that can be automated?

Hi everybody. I have spheroids' images that I want to analyze in respect to lumen area, spheroid area and number of nuclei. I generated a script in image J to automate my analysis, but it just...

02 April 2021 4,773 4 View

How dissolve forskolin and prevent its precipitation?

I am treating cells with 10µM and 100µM forskolin ( the stock solution is dissolved in DMSO). But whenever I dilute it and add it to the media it precipitates forming crystals. Anybody knows what...

10 April 2019 5,959 1 View

How to install Color Pixel counter Plugin in image J?

I am trying to count number of pixels in an image and have a trouble installing the Color Pixel counter plugin in image J. I know the steps of downloading the plugin and copying it to Plugin file...

30 April 2018 3,378 2 View

Number of cells needed for Proteome Profiler Array?

I will be determing the expression of apoptosis proteins in MDA-MB-231 cells upon drug treatment. What is the optimal number of cells usually used to get good protein concentration?

12 April 2018 5,446 3 View