Fatima Hassan

9 Questions 4 Answers 0 Followers

Questions related from Fatima Hassan

Can SRC kinase inhibitor (PP2) increase pSRC levels?

)Hi all, I tried to inhibit pSRC downstream signalling by using SRC kinase inhibitor PP2. In my experiments I could detect a reduction in protein levels downstream SRC, However, SRC...

28 February 2022 1,167 3 View

What are the reasons for unsuccessful ligation in a pJET1.2 cloning vector?

I am trying to sequence alleles by ligating short PCR products into a pJET 1.2 vector. Although pJET protocol states that the plasmid contains suicidal genes that are disrupted once ligation is...

31 January 2022 5,829 1 View

What is a good image analysis software that can be automated?

Hi everybody. I have spheroids' images that I want to analyze in respect to lumen area, spheroid area and number of nuclei. I generated a script in image J to automate my analysis, but it just...

02 April 2021 4,789 4 View

What causes precipitates in a collagen gel assay?

I seeded MDCK cells in collagen in order to form spheroids, since seeding I saw a massive amount of precipitates (images attached) making it so hard to visualize the seeded cells. Does anybody...

07 January 2021 5,816 0 View

How to induce lumen formation in spheroids in 3D culture?

Hi all, I am using human kidney immortalized cells, these cells form spheroids in matrigel in 3D culture, but do not form any lumens. Is there a way to induce lumen formation in spheroids?

20 May 2020 3,503 0 View

How dissolve forskolin and prevent its precipitation?

I am treating cells with 10µM and 100µM forskolin ( the stock solution is dissolved in DMSO). But whenever I dilute it and add it to the media it precipitates forming crystals. Anybody knows what...

10 April 2019 5,963 1 View

How to install Color Pixel counter Plugin in image J?

I am trying to count number of pixels in an image and have a trouble installing the Color Pixel counter plugin in image J. I know the steps of downloading the plugin and copying it to Plugin file...

30 April 2018 3,384 2 View

Number of cells needed for Proteome Profiler Array?

I will be determing the expression of apoptosis proteins in MDA-MB-231 cells upon drug treatment. What is the optimal number of cells usually used to get good protein concentration?

12 April 2018 5,451 3 View

What is the difference between MTT assay and WST assay?

What is the difference between MTT and WST assays?

18 March 2017 8,827 1 View