Akash Sabarwal

10 Questions 76 Answers 0 Followers

Questions related from Akash Sabarwal

How can we isolate, characterise and maintain cancer stem cells from lung cancer cells lines?

From A549 and H1299 cells, After isolation which media can be used to maintain cancer stem cells?

03 March 2018 3,222 7 View

How much old protein lysates can be used for western blotting?

Stored at -20 or -80 degrees.

10 October 2016 7,298 3 View

Debris in cell culture ?

I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the...

10 October 2016 6,347 10 View

There are different ROS which are produced in cultured cells, DCFDA and DHE both stains and detect these ROS, What are the differences?

How DHE and DCFDA detects ROS differently?

10 October 2016 4,641 4 View

What are other antibiotic solutions available for cell culture apart from Streptomycin/Penicillin?

What are other antibiotics used and available commercially for animal cell culture

10 October 2016 8,546 3 View

How the concentration of CO2 may effect the growth of cultured cells?

How important Co2 concentration is for cultured cells

10 October 2016 3,371 1 View

Can we use Caco 2 cells as normal cells for toxicity testing of a chemical?

We don not have normal cells, can we use Caco 2 cells instead

09 September 2016 2,991 4 View

In which cancer, conventional chemotherapy is least beneficial?

With respect to Organs

09 September 2016 8,084 0 View

What are the best time points to study epigenetic regulation?

For proteins such as HDACs and DNMSts

07 July 2016 9,569 2 View

How can I induce inflammation in gastric cancer cells using LPS?

Please tell which LPS (strain of E.coli) would give best results. Time of induction? what about starvation condition ? I tried with 100ng/ml to 4 ug/ml but could'nt get COX 2 or cCaspase 1?

06 June 2015 1,552 3 View