How to convert frequency-amplitude data to time-amplitude data using inverse fourier transform?

Hello, I have data in an Excel spreadsheet that is in terms of frequency and amplitude. I want to convert this into time and amplitude using an inverse Fourier transform. What script could I use...

30 April 2020 4,591 4 View

MD simulation data using MDplot in R packages?

I have been trying load my MD data on MDplot. Any suggestions on how to use MDplot for plotting Gromacs 5.0.1 generated MDS data ?

31 January 2019 8,214 5 View

Problem with L929 cells?

After 3days of splitting I am facing turbidity problem in L929 cells(Fibroblasts),But there are no symptoms of bacterial contamination.. Some protocol says to isolate conditioned medium from L929...

09 October 2016 4,451 2 View

Can we harvest energy from electric and magnetic field components of an electromagnetic wave respectively and store them ?

its just regarding the wireless energy transmission, and considering that light is an electromagnetic wave.

30 August 2016 7,281 10 View

How can I decompose in a bandstop FIR filter with a broad stopband and two narrow passbands at wt=0 and wt=pi as a sum of 2 lateral filters?

how to evaluate the lateral filters with minimal value of maximum ripple in their stop bands.

29 December 2014 4,329 14 View

How do ceramic nanoparticles work as antiwear additives in lubricated condition?

19 November 2014 9,891 1 View

Has anyone used DH5 alpha for in-fusion cloning?

Is it possible to use DH5 alpha for in-fusion cloning if the insert (2 kb) and the vector (11.7 kb) are this large?

15 June 2014 5,298 3 View

How to get PID tuning parameters with this code?

Codes in MATLAB for Particle Swarm Optimization How to get the PID tuning parameters with this code? What are the changes have to be done?

01 January 1970 8,704 1 View

Image segmentation using matlab

Can anyone give the MATLAB code for foreground detection using MATLAB?

01 January 1970 5,574 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View