what are the best kits for enrichment and Isolation kits for of Circulating tumor cells? What hurdles can I expect while handling CTCs from whole blood? Request your experiences.
Hi Kalyani,
in our lab we are working with two different systems for enrichment: CellSearch (Menarini) and Parsoritx (Angle). They all have pros and cons, but CellSearch also give you a CTC count, while Parsortix doesn't have this option. There are new technology coming up from different companies that will include the "counting option" as well (probably as an extra).
After this, for CTC identification and isolation we use the DEPArray (Menarini).
Main problem of the whole process is low recovery. Your samples should contain a high number or CTCs to be able to recover them. Other problems, it is time consuming when processing the images before elution of single cells or pools, as images requires visual verification (you could face several problems if don't verify).
Other problems linked to single CTC analysis are the WGA you need to run afterwards, in case you want to sequence them. There are several systems to do that, but none of the is perfect. In our lab, we are using the Ampli1 from Menarini.
Hope this all helps.
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