Can someone suggest hybridization buffer protocol FISH, and best protocol for good hybridization of probes, please??

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Where can I access the relation between environmental humidity range and disease infestation in agricultural field?

Different disease (fungal and bacterial) are triggered by specific range of relative humidity but its somehow tough to collect all the information searching for each disease. Are there any...

24 September 2023 6,849 5 View

How is damage scoring of Red Pumpkin Beetle done and its control threshold calculated?

I have been reviewing various journals for the visual leaf as well as fruit damage scoring of Red Pumpkin Beetle (Aulacophora foveicollis). It would be really helpful if any of you have some...

14 October 2022 7,531 1 View

TCGABiolinks: Error in strsplit(c(colnames(data)), “-”) : non-character argument #459?

Hi, I am working with the new CMI-MBC data and was using TCGABiolink for DEGs identification.. Now at step ''' dataDEGs

23 April 2021 3,329 0 View

Jupyter/Python code to directly retrieve sequence data from Uniprot?

I am trying to work on Uniprot directly from my jupyter notebook. My search keywords are TERM "tumor+necrosis+factor+receptor" and ORGANISM "Homo sapiens". Here's how far I have succeeded import...

25 March 2021 6,774 5 View

How to convert frequency-amplitude data to time-amplitude data using inverse fourier transform?

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30 April 2020 4,718 4 View

best DNA Isolation kits for Circulating tumor cells? What would be the pros and cons while handling CTCs from whole blood from ur experi?

what are the best kits for enrichment and Isolation kits for of Circulating tumor cells? What hurdles can I expect while handling CTCs from whole blood? Request your experiences.

26 January 2020 3,452 2 View

MD simulation data using MDplot in R packages?

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31 January 2019 8,287 5 View

Is there anything called inverse tensor product of 2 simple undirected graphs?

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13 November 2017 683 2 View

Why soxhlet apparatus is used in extarction of plant material??

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06 July 2017 5,345 7 View

Can someone provide ieee 118 bus, 19 machine dynamic data ?

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22 March 2017 6,311 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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06 August 2024 726 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Mert Sudagidan

Dear Kalyani, the following protocol books can help your studies.

Best regards.

Springer Protocols Handbooks

Fluorescence In Situ Hybridization (FISH): Application Guide [2 ed.]

Methods in Molecular Biology 1211

In Situ Hybridization Protocols [4 ed.]

Kalyani Palasamudram

Thank you for the suggestions Mert.