How to store a nitrocellulose membrane after western blot?

01 February 2020 9,382 3 View

How to reduce non-specific interaction without impairing antibody-epitope binding in immunohistochemistry (IHC) ?

Dear expertise, i stained frozen section lung tissue sample against virus protein, which should be detect virus infection. i trying to reduce non-specific interaction using serum (5% BSA in PBS)...

07 August 2019 9,401 2 View

What are the criteria need to apply post-doctoral position ?

Dear Expert, I would like to apply postdoctoral studies. 1. I am wondering how to apply for postdoctoral position especially in the US and Europe school. What are the procedures, I need to go...

06 July 2018 1,022 5 View

What are the OIE- Listed bacterial diseases mostly infecting the shrimp worldwide?

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03 April 2017 6,945 1 View

Could anybody share their protocol for Mycobacterium bacteriophage isolation from aquatic environment?

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03 April 2017 9,679 1 View

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I have done aquatic animal model experiments, which are treated with single, combination of different bacteriophage under laboratory condition, and I controlled bacterial infection by phages. I...

08 September 2016 8,024 11 View

Does anyone know where the facility of Pulsed-field gel electrophoresis for DNA sample in India?

05 June 2016 10,052 1 View

How do I concentrate the PCR products ?

10 November 2015 3,632 25 View

What is the basic method for bacteriophage SDS-PAGE and How do i get all phage structural protein bands ?

1. I am getting streaking with two big bands, two normal bands two/ three very small bands only between 70-40 kDa in 12 % SDS-PAGE gel (CBB strain). but i unable to remove streaking. Is there any...

08 September 2015 4,827 2 View

How to screen out for GAL gene containing bacteriophage from the plagues ?

03 April 2015 556 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View