If made 400L of MEM 199 medium and added 8 L of extra water?

09 October 2016 2,259 3 View

What is the difference in the use of Trypsin 0.25% vs Trypsin /EDTA 0.25% 1mM EDTA for tissue culture ?

03 April 2016 5,436 4 View

How do you perform a flow cytometry experiment on DC maturation of pig tonsil tissue, and what markers do you need?

I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...

02 March 2015 6,026 2 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultraviolet UV irradiation?

Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?

31 December 2013 7,880 0 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultra violet UV irradiation?

QPCR to UV treated rubella RNA.

11 December 2013 3,909 0 View

In the biotech industry regarding stability of viral antigen, what are the factors that can effect the stability of the product?

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01 February 2013 1,037 1 View

I want to produce Salmon pancreatic virus

Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks

09 October 2010 5,292 0 View

Any can give me Information regarding the use of PERFULOROPOLY ETHER

I need to How PERFULOROPOLY ETHER works to seprate proteins?

03 April 2010 9,610 0 View

Viruses Concentration

Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank

02 March 2010 5,812 0 View

Hbe Ag Production methods

Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact

01 February 2010 8,537 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View