12 Questions 17 Answers 0 Followers
Questions related from Asim Shaghil
I made 400 L of MEM 199 medium (sigma powder cat M0393 Medium 199 ) and added extra 8 L of water. Some people saying that i over diluted this media and it wont be good. Please give your opinion....
10 October 2016 2,289 3 View
Why and what type of cell lines we use these type of trypsin 1. Trypsin 0.25% vs 2. Trypsin /EDTA 0.25% 1mM EDTA 3. types of cells line we use above mentioned trypsins
04 April 2016 5,473 4 View
I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...
09 September 2015 4,000 1 View
I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...
03 March 2015 6,090 2 View
Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?
01 January 2014 7,922 0 View
QPCR to UV treated rubella RNA.
12 December 2013 3,948 0 View
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02 February 2013 1,074 1 View
Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks
10 October 2010 5,322 0 View
I need to How PERFULOROPOLY ETHER works to seprate proteins?
04 April 2010 9,653 0 View
Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank
03 March 2010 5,852 0 View
Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact
02 February 2010 8,578 4 View
Hi, I need to produce HBe Ag would ney one please know what cell lines are the best to work for HBe Ag prosuction and any one have the method or research citation as well. please mentor me
02 February 2010 4,070 0 View
