If made 400L of MEM 199 medium and added 8 L of extra water?

09 October 2016 2,259 3 View

How to coat porcine saliva on Maxisorp 96 well plate?

I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...

08 September 2015 3,930 1 View

How do you perform a flow cytometry experiment on DC maturation of pig tonsil tissue, and what markers do you need?

I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...

02 March 2015 6,026 2 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultraviolet UV irradiation?

Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?

31 December 2013 7,880 0 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultra violet UV irradiation?

QPCR to UV treated rubella RNA.

11 December 2013 3,909 0 View

In the biotech industry regarding stability of viral antigen, what are the factors that can effect the stability of the product?

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01 February 2013 1,037 1 View

I want to produce Salmon pancreatic virus

Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks

09 October 2010 5,292 0 View

Any can give me Information regarding the use of PERFULOROPOLY ETHER

I need to How PERFULOROPOLY ETHER works to seprate proteins?

03 April 2010 9,610 0 View

Viruses Concentration

Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank

02 March 2010 5,812 0 View

Hbe Ag Production methods

Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact

01 February 2010 8,537 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View