What is the difference in the use of Trypsin 0.25% vs Trypsin /EDTA 0.25% 1mM EDTA for tissue culture ?

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Zia Mohammed shabbir

Calcium (Ca2+ ) and Magnesium ions(Mg2+)INHIBIT TRYPSIN. EDTA is a Calcium chelator which will "mop" up the remaining divalent cations.

Asim Shaghil

Thanks Shabbir, my question is what its role during trypsinisation of cells and how it differs from non EDTA based trypsins?

with EDTA by chelating divalent cationic inhibitors of trypsin, increases the efficacy of trypsin in isolating cells

Thanks a lot Shabbir