If made 400L of MEM 199 medium and added 8 L of extra water?

09 October 2016 2,259 3 View

What is the difference in the use of Trypsin 0.25% vs Trypsin /EDTA 0.25% 1mM EDTA for tissue culture ?

03 April 2016 5,436 4 View

How to coat porcine saliva on Maxisorp 96 well plate?

I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...

08 September 2015 3,930 1 View

How do you perform a flow cytometry experiment on DC maturation of pig tonsil tissue, and what markers do you need?

I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...

02 March 2015 6,026 2 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultraviolet UV irradiation?

Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?

31 December 2013 7,880 0 View

In the biotech industry regarding stability of viral antigen, what are the factors that can effect the stability of the product?

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01 February 2013 1,037 1 View

I want to produce Salmon pancreatic virus

Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks

09 October 2010 5,292 0 View

Any can give me Information regarding the use of PERFULOROPOLY ETHER

I need to How PERFULOROPOLY ETHER works to seprate proteins?

03 April 2010 9,610 0 View

Viruses Concentration

Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank

02 March 2010 5,812 0 View

Hbe Ag Production methods

Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact

01 February 2010 8,537 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View