Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank
I made 400 L of MEM 199 medium (sigma powder cat M0393 Medium 199 ) and added extra 8 L of water. Some people saying that i over diluted this media and it wont be good. Please give your opinion....
09 October 2016 2,319 3 View
Why and what type of cell lines we use these type of trypsin 1. Trypsin 0.25% vs 2. Trypsin /EDTA 0.25% 1mM EDTA 3. types of cells line we use above mentioned trypsins
03 April 2016 5,508 4 View
I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...
08 September 2015 4,039 1 View
I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...
02 March 2015 6,118 2 View
Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?
31 December 2013 7,957 0 View
QPCR to UV treated rubella RNA.
11 December 2013 3,983 0 View
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01 February 2013 1,104 1 View
Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks
09 October 2010 5,347 0 View
I need to How PERFULOROPOLY ETHER works to seprate proteins?
03 April 2010 9,685 0 View
Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact
01 February 2010 8,607 4 View
Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...
07 May 2024 259 3 View
Hi follow virologists, I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased...
18 March 2024 5,174 4 View
Dear virologists; What is the PCR technique used in virology to detect viral nucleic acids? The steps involved. I'd also like to know, since some viruses have a single strand of DNA and RNA, how...
28 February 2024 1,869 10 View
Is it possible that a hybrid virus is generated in a eukaryotic cell by co-infection of different viruses through nonhomologous recombination?
14 September 2023 7,245 3 View
For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...
22 August 2023 1,600 1 View
I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.
26 July 2023 7,550 1 View
Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...
29 May 2023 7,105 6 View
Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...
17 April 2023 5,873 6 View
I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...
12 January 2023 4,248 2 View
Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.
27 November 2022 9,614 2 View