If made 400L of MEM 199 medium and added 8 L of extra water?

09 October 2016 2,259 3 View

What is the difference in the use of Trypsin 0.25% vs Trypsin /EDTA 0.25% 1mM EDTA for tissue culture ?

03 April 2016 5,436 4 View

How to coat porcine saliva on Maxisorp 96 well plate?

I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...

08 September 2015 3,930 1 View

How do you perform a flow cytometry experiment on DC maturation of pig tonsil tissue, and what markers do you need?

I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...

02 March 2015 6,026 2 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultraviolet UV irradiation?

Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?

31 December 2013 7,880 0 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultra violet UV irradiation?

QPCR to UV treated rubella RNA.

11 December 2013 3,909 0 View

I want to produce Salmon pancreatic virus

Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks

09 October 2010 5,292 0 View

Any can give me Information regarding the use of PERFULOROPOLY ETHER

I need to How PERFULOROPOLY ETHER works to seprate proteins?

03 April 2010 9,610 0 View

Viruses Concentration

Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank

02 March 2010 5,812 0 View

Hbe Ag Production methods

Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact

01 February 2010 8,537 4 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View