In the biotech industry regarding stability of viral antigen, what are the factors that can effect the stability of the product?

02 February 2013 1 1K Report

The method of purification of the antigen plays a key role, apart from that the temperature susceptibility, the nature of the protein molecule(antigen) will be my opinion Sir.

Badges
Science topic

More Asim Shaghil's questions See All

If made 400L of MEM 199 medium and added 8 L of extra water?

I made 400 L of MEM 199 medium (sigma powder cat M0393 Medium 199 ) and added extra 8 L of water. Some people saying that i over diluted this media and it wont be good. Please give your opinion....

09 October 2016 2,319 3 View

What is the difference in the use of Trypsin 0.25% vs Trypsin /EDTA 0.25% 1mM EDTA for tissue culture ?

Why and what type of cell lines we use these type of trypsin 1. Trypsin 0.25% vs 2. Trypsin /EDTA 0.25% 1mM EDTA 3. types of cells line we use above mentioned trypsins

03 April 2016 5,508 4 View

How to coat porcine saliva on Maxisorp 96 well plate?

I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...

08 September 2015 4,039 1 View

How do you perform a flow cytometry experiment on DC maturation of pig tonsil tissue, and what markers do you need?

I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...

02 March 2015 6,118 2 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultraviolet UV irradiation?

Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?

31 December 2013 7,957 0 View

Can I amplify or quantitate by using qPCR to rubella Virus RNA after ultra violet UV irradiation?

QPCR to UV treated rubella RNA.

11 December 2013 3,983 0 View

I want to produce Salmon pancreatic virus

Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks

09 October 2010 5,347 0 View

Any can give me Information regarding the use of PERFULOROPOLY ETHER

I need to How PERFULOROPOLY ETHER works to seprate proteins?

03 April 2010 9,685 0 View

Viruses Concentration

Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank

02 March 2010 5,881 0 View

Hbe Ag Production methods

Hi, I am looking some one who is more experienced in HBe ag production? I need some mentor in this regard. Please contact

01 February 2010 8,607 4 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

Why not use CD11b staining to evaluate the purity of primary microglia?

I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?

02 July 2024 9,411 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

How do you bleed HIV-infected humanized mice?

Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...

27 June 2024 7,707 2 View