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Good morning everyone. I would like to rescue the virus from clones. Now I have 4 inserts of DNA. DNA 1: ~4.5kb (digested by AscI/PacI) DNA 2: ~4.7kb (digested by PacI/KpnI) DNA 3: ~4.5kb...
09 September 2025 2,438 1 View
Hello Everyone, Can BSSE correction energy be negative? I mean, when we apply the BSSE correction, can the resulting energy be negative — for example, -0.03 — or must it always be positive?"
07 September 2025 9,488 0 View
As I worked with small IR sensors since 2016, I produces a controller board to control every Dc or ac motor with every current and I tested electrical board in hand dryer and liquid soap dropper...
05 September 2025 3,948 0 View
I know that the lentiviral packaging method has a good efficiency, but i have no idea about viral versus bacterial plasmid efficiency using transfection, not transduction. I think these methods...
04 September 2025 4,948 4 View
ABOUT PARADIGM DEATH AND SHIFT Are you familiar with the idea a la Thomas Kunn where when there is a paradigm shift the old paradigm and all of its supporting and supported parts are left behind,...
31 August 2025 4,243 2 View
Hello everyone, I am currently working on a sandwich ELISA and have encountered an issue I’d like some insight on. My assay is consistently showing higher signal in the non-diseased control serum...
29 August 2025 1,340 2 View
Hello, I was looking around for a concentration of pronase to break down tissue in human pancreas. I would appreciate whatever values you find as well as how to prepare it. Thank you!
29 August 2025 5,340 1 View
How can CRISPR-mediated targeted knock-in achieve gene expression levels more than ten times higher than the baseline? Literature reports that lentiviral infection followed by screening for...
26 August 2025 2,126 0 View
I am currently performing a plasmid construction based on T4 DNA ligase. I first designed primers to incorporate identical restriction sites at both the 3' and 5' ends of the target fragment. This...
22 August 2025 9,887 1 View
What Information Should You Include in the Methodology?
20 August 2025 2,142 2 View
I want to treat cancer cell lines with a treatment, and I want to calculate IC50. Should my cells after 24 hr of seeding be confluent or what is the suitable percentage that cells should be?
12 August 2025 6,427 7 View
If you are aware of any solid references or studies to recommend, or if you have conducted such an assessment yourself, I would greatly appreciate your input. Thank you in advance for your...
06 August 2025 9,644 2 View
Medical professionals suspect perforation and bleeding more in elderly people with short life expectancies.
05 August 2025 9,459 2 View
Hi everyone! Does anyone made a puromycin curve for the cells lines Hepa1-6, MC38, SW948 and LS174T where was observed 100% cell death on days 5 and 7? Thanks in advance, Kind regards
04 August 2025 6,252 0 View
Hello All, I am currently optimising an SOP for cytospin using cell lines but am observing some aggregates on my slides (after cytospin) that I want to avoid. I use disposable cytofunnels,...
04 August 2025 9,125 2 View
Pregunta enfocada en programas de apoyo emocional en centros educativos, uso de apps de autocuidado, trabajo grupal y el rol del entorno familiar y comunitario como factores protectores.
09 July 2025 7,729 0 View
My research explores the therapeutic potential of video games as a developmentally appropriate and engaging tool within play therapy and behavioral health settings. Drawing from my experience as a...
07 July 2025 2,567 1 View
How individual happiness is affected by the happiness management of parents, workplaces, government and the self?
02 July 2025 5,543 1 View
I wonder if there is any journal with no Article Processing Charge to publish bibliometric study related article.
27 June 2025 8,263 0 View
I have prepared some plant-based aqueous extracts, and I would like to perform a comprehensive analysis of their composition. Would it be convenient to freeze-dry the extracts and then resuspend...
19 June 2025 5,753 7 View
I am encountering possible issues of toxicity due to expression of eGFP in my cell line, introduced using a lentiviral vector. Wondering if anyone has experience using lentivirus to induce...
11 June 2025 4,328 6 View
Hi everyone! Unfortunately my chemical has not arrived but I have already seeded (20,000 cells/wells) the cells, so I would like to know that how long can we keep SH-SY5Y cell line in 96-well...
27 May 2025 5,210 4 View
We purchased the Keithley 2601A SourceMeter and lost the installation software. Can we find a researcher who has the same meter to share the software with us? Thank you.
27 May 2025 2,966 0 View
En la práctica empresarial el enfoque estratégico suele llevarse de manera independiente al control interno. Aun cuando en la elaboración de los planes estratégicos se tienen en cuenta mecanismos...
20 May 2025 9,181 2 View