Hi,

I did matrigel assay a while ago and it worked. Are you using serum free medium for dilution?

I diluted original matrigel (10-12mg/ml, BD catalog number 354234) to 0.7mg/ml with serum-free DMEM medium,

Applied 200 ul to upper chamber of Transwell, leave 2hr at Room temp,

It worked well, we did publish too,

Hope this helps

I used plain stock media for dilution. Did you use 24-well inserts? Because 200ul seems like a lot for that. Also, Which cells did you use? 0.7mg/ml is much less concentrated than anything I've tried.

When you added your cells did the matrigel still move like a liquid if you tilted the plate or was it cemented in place?

For migration/invasion studies usually we make a mix of matrigel/collagen/fibronectin, as it represents tumor microenvironment in vivo better. Matrigel final concentration usually is about 2 mg/ml, ant this mix solidifies quite well at 37C in incubator (in about 20-30 mins). Fibronectin helps cells to attach to it during migration.

Hello. In my case it happens the same. I take matrigel to a dilution of 1mg/ml or 2,5mg/ml but it still continues in a liquid state. I really don't know how to gel it. I follow the whole protocol but diluted matrigel never gels.