22 Questions 778 Answers 0 Followers
Questions related from Subhash C. Juneja
What are best models in mouse to study glaucoma? Is intracameral injections of beads a good model?
26 April 2023 843 0 View
Hi I want to program C1000 for touchdown PCR protocol (pl see enclosed file) If someone can help me how to enter it in C1000 machine Thanks
05 February 2023 2,270 0 View
One of my close relative has adrenal adenoma on one side and kidney disease also. Will unilaterlal adrenalectomy improve kidney functions or worsens kidney functions ?
17 June 2018 2,484 8 View
Normally tissue is fixed in 4% PFA (or animal perfused with 4% PFA before taking tissue from animal), than the tissue passed thru 15% sucrose and than in 30% sucrose before freezing in OCT blocks....
16 June 2018 499 6 View
In tamoxifen-induced Cre-ER system, why they use mutated form of ligand binding domain estrogen receptor?
28 June 2017 6,998 2 View
Scientist has used Cleaved Caspase 3 to determine apoptosis. Also they have used Caspase3/7 assay for apoptosis. I am wondering if in "Caspase3/7 assay", the Caspase 3 and Caspase 7 are cleaved....
16 January 2017 6,886 3 View
3% H2O2 is mentioned in most of papers which normally works good in my case. But some have used additionally 100% methanol, Does methanol help? Some companies (DAKO) sell Endogenous Blocking...
26 October 2015 3,755 1 View
Normally healthy articular cartilage express Type II Collagen and no Type I collagen. As based on reports in literature, People have shown the expression of Type I collagen besides Type II...
17 September 2015 1,614 16 View
I can see lipid droplets in chondrocytes in ex vivo chondrogenesis from stem cell differentiation (a figure enclosed). Report does exist in literature that lipid droplets are present in human...
04 June 2015 1,994 11 View
I am looking for simple way to detect human tissues implanted in athymic rodent. If anyone has used the anti-nuclei antibody or other antibody, please let me know how it worked?
16 January 2015 9,830 13 View
Some studies use fluorescent-labelled cells for transplantation in knee or in any bone for tracing the fate of transplanted cells: I really want to know if the fluorescence is lost due to...
30 September 2014 7,909 2 View
Biomaterial or scaffold for stem cells to transplant in osteo-chondral defects in rat or rabbit. There are large number of biomaterial experts are out there on RG site. My stem cells placed in...
01 September 2014 2,402 9 View
Wondering if someone has done X-gal staining on decalcified bone section in rat or mouse. My fear is that if decalcification process would interfere with X-gal staining, Thanks,
21 August 2014 3,929 5 View
What is the best protocol for decalcification of rat knee suitable for immunohistochemistry, safranin-O (cartilage) and TRAP staining (bone remodelling) on paraffin-sections. Please share your...
16 July 2014 4,004 11 View
Thanks in advance.
09 July 2014 1,749 9 View
Anyone wants to share their experience of MSCs differentiation into articular cartilage and growth plate cartilage, and exact growth factors used for either type, i.e, Tgb and Bmp
08 July 2014 4,539 6 View
Synovial Fibrosis Does anyone have experience in synovial fibrosis in surgically-induced OA rat knee or mouse or other animal model? Has anyone tried to reduce synovial fibrosis while trying to...
17 May 2014 4,528 2 View
There are beliefs that iliac crest bone marrow provides superior MSCs than femur does. I collected bone marrow of the femur from patients undergoing TKR or hip surgery. Stem cells isolated from...
27 February 2014 4,037 10 View
I need to test chondrocytes for their in vivo ability to repair articular cartilage defects in rat femoral patellar groove. Need to know the depth of defect I should create.
21 February 2014 6,321 5 View
Literature has shown some papers of in vitro chondrogenesis on Collagen II-coated inserts. Since in vivo cartilage is on bone and bone is rich in Collagen-I, wouldn't it be a better model? Any...
30 January 2014 9,323 2 View
Histology of induced osteoarthritic knee of rat
24 January 2014 2,867 2 View
Though Tgfb1 and Tgfb3 play different roles in fibrosis and wound healing, there is no difference in their chondrogenic ability and nature of cartilage they induce from MSCs. Can anyone elaborate...
22 January 2014 4,283 2 View
