Subhash C. Juneja

6 Questions 457 Answers 0 Followers

Questions related from Subhash C. Juneja

Why we soak tissues in 30% sucrose prior to embedding in OCT media?

Normally tissue is fixed in 4% PFA (or animal perfused with 4% PFA before taking tissue from animal), than the tissue passed thru 15% sucrose and than in 30% sucrose before freezing in OCT blocks....

16 June 2018 424 6 View

Why to use mutated form of ligand binding of estrogen receptor in Cre-ER cassette?

In tamoxifen-induced Cre-ER system, why they use mutated form of ligand binding domain estrogen receptor?

28 June 2017 6,941 2 View

Has anyone used Anti-Nuclei Antibody, clone 235-1 from Millipore to detect human cells in rat or mouse tissues?

I am looking for simple way to detect human tissues implanted in athymic rodent. If anyone has used the anti-nuclei antibody or other antibody, please let me know how it worked?

16 January 2015 9,762 13 View

Does anyone have knowledge on the effect of decalcification on fluorescence?

Some studies use fluorescent-labelled cells for transplantation in knee or in any bone for tracing the fate of transplanted cells: I really want to know if the fluorescence is lost due to...

30 September 2014 7,853 2 View

What is the optimum protocol of decalcification of rat bones?

What is the best protocol for decalcification of rat knee suitable for immunohistochemistry, safranin-O (cartilage) and TRAP staining (bone remodelling) on paraffin-sections. Please share your...

16 July 2014 3,926 11 View

Why aren't Collagen I-coated inserts represented as a better model than Col-II coated bed for in vitro chondrogenesis?

Literature has shown some papers of in vitro chondrogenesis on Collagen II-coated inserts. Since in vivo cartilage is on bone and bone is rich in Collagen-I, wouldn't it be a better model? Any...

30 January 2014 9,256 2 View