Tyler Panzner

3 Questions 3 Answers 0 Followers

Questions related from Tyler Panzner

Has anyone had success with antibody conjugation kits for ICC/IHC studies?

I am planning a multiplex ICC experiment. The issue is that several of my antibodies are anti-rabbit which would cross react with secondary antibodies. I was wondering if anyone has used antibody...

24 March 2020 6,333 3 View

Wrong Band Sizes for Gelatin Zymography?

Attached is my gelatin zymography for 4T1 breast cancer cells. I don't see any banding for MMP2 (around 65-72 kDA) or MMP9 (around 85-92 kDA). In my opinion, the bands above 100 kDa are MMP...

19 May 2018 2,656 6 View

How "solid" should solidified Matrigel be/matrigel concentrations?

I am trying to perform a matrigel boyden chamber invasion assay with breast cancer cells. However, when I used my lab's protocol and diluted the matrigel to 1.8mg/ml, it never seems to solidify...

19 May 2018 5,724 4 View