Fernando Laguía Nueda

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Questions related from Fernando Laguía Nueda

Does anyone have a protocol about growing 3D culture in matrigel in order to extract protein for Western Blot assay?

Hello. I'd like to perform a Western Blont with samples obtained from 3D culture in matrigel. I've done experiments with 3D cultures in 96 well plates in order to test drug activity, but now I'm...

28 July 2020 1,594 4 View

How can I calculate synergistic effect without getting an IC50 value for individual drug treatment?

Hello! I need to calculate synergistic effect of 2 drugs, however, when I treat my cell culture with them, I can't get an IC50 value, as I can't use a dose higher than 10uM following...

20 March 2019 2,794 6 View