How much IL-10 secreted from 1million (isolated) CD4+ cells?

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Protocol for fixing and mounting fungi from agar culture?

I'm looking for a good protocol for taking fungi (mostly conidial ascomycetes) growing on an agar plate and fixing them and ultimately staining and mounting them. Would one simply remove a plug of...

03 June 2019 8,227 5 View

Gelatin as a gelling agent for fungal growth media?

I'm planning an experiment involving imaging cultures of mycoparasitic Trichodermium interacting with various species of host fungi and am thinking of various methods of imaging the interaction...

26 May 2019 2,466 3 View

How to alter dimer-monomer equilibrium in proteins?

I have a protein forming a very stable dimer in solution, but still running as monomer in SDS page. I have tryed to disrupt the dimer with Beta-ME and high salt, but it is still running as dimmer...

21 January 2018 2,921 3 View

Can a short burst of electricity cause irreversible membrane damage to Ecoli strains?

No growth observed on plates and I can't explain why as the sample was only subjected to current for a second.

10 November 2017 4,016 1 View

Experience with Foxp3 intracellular staining?

Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...

03 January 2017 8,353 5 View

Anti-CD3 for stimulation is displaced?

I have been activating T cells with anti-CD3 (either soluble or plate bound) for many years. Currently, I am establishing a protocol whereby at the time of coating (or subsequently) I plate bind...

19 October 2016 4,989 1 View

I am getting different results from DLS measurements , what kind of errors I might have or what can be the reason ?

09 June 2016 2,614 5 View

How can I transform a data series with negative, zero, and positive data points?

The data series is from changes in bee hive weights per day, so all data points are important. They are not normally distributed (many zeros) hence the need to transform the series. I wanted to...

01 June 2016 7,183 7 View

What are the best resources to aid in determining what questions to ask in a survey?

Survey resources focus on what surveys are and how they are constructed but does not provide guidance on how or what to translate from literature review or other research inputs into a survey. Are...

10 January 2016 7,384 10 View

Appropriate statistical test for Likert items and scales to find out if there is any significant difference in the responses of 3 different groups?

Is Pearson's chi-sqaure test appropriate to use for Likert items? I want to find out whether there is any difference in the responses from 3 different groups? If it is Kruskal-Wallis Test, how...

17 November 2015 4,319 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View